d) What would happen to the Rr of the spots if you increased the polarity of the mobile phase? Explain

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter34: Miscellaneous Separation Methods
Section: Chapter Questions
Problem 34.1QAP
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please answer part d

3. The main components of clove oil are eugenol, eugenol acetate, and b-caryophyllene.
но
eugenol
Eugeoohacetate
B-caryophyllene
a) Rank the compounds from least polar to most polar
b) After analyzing clove oil by TLC using a 10% acetone in hexanes mobile phase, you obtain the following
TLC results. Identify the compound that corresponds with each spot.
5 cm
2.8 cm
c) Calculate the Rr value of the middle spot. Show your work
d) What would happen to the Rr of the spots if you increased the polarity of the mobile phase? Explain
e) When separating the compounds by column chromatography, which compound would you expect to elute
first? Which compound would you expect to elute last?
Transcribed Image Text:3. The main components of clove oil are eugenol, eugenol acetate, and b-caryophyllene. но eugenol Eugeoohacetate B-caryophyllene a) Rank the compounds from least polar to most polar b) After analyzing clove oil by TLC using a 10% acetone in hexanes mobile phase, you obtain the following TLC results. Identify the compound that corresponds with each spot. 5 cm 2.8 cm c) Calculate the Rr value of the middle spot. Show your work d) What would happen to the Rr of the spots if you increased the polarity of the mobile phase? Explain e) When separating the compounds by column chromatography, which compound would you expect to elute first? Which compound would you expect to elute last?
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