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Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli
b. Pseudomonas aeruginos
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- Describe what the plate in each scenario looks like: A. Bacteria grows on mannitol salt agar and is negative for mannitol fermentation B. Bacteria grows on EMB agar and is positive for lactose fermentation C. Bacteria grows on MacConkey agar and is negative for lactose fermentationWhy can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agarIf you streak a sample from your nose onto a Mannitol salt agar plate and the medium turns yellow, what does that tell you? (choose all that apply) a. The organism is a yeast b. The organism ferments mannitol c. The organism is beta-hemolytic d. The organism is likely Staphylococus aureus e. The organism is acid tolerant
- MacConkey (MAC) Agar is used to isolate gram-negative enteric bacteria.Peptone provides nutrients necessary for growth.Bile salts and crystal violet inhibit the growth of gram positive bacteria.Lactose is a carbohydrate. When metabolized, colonies turn the medium pink.Which organism/s will grow in the medium? a. Escherichia coli b. Pseudomonas aeruginosa c. Staphylococcus aureus d. Escherichia coli & Pseudomonas aeruginosa e. Pseudomonas aeruginosa & Staphylococcus aureuscommercial sterilization is designed to provide enough heat to destroy the endospores of what organism? a. clostridium tetani b. stapylococcus aureus c. prions d. bacillus anthracis e. clostridium botulinumWhat is the difference between the use of Peppermint oil and Cinnamon oil in Veterinary Medicine to treat the bacterial infections Escherichia coli and Staphylococcus?
- How can one distinguish E. coli from P.aeruginosa on nutrient agar? blood agar? EMB agar? What is the major difference between Thayer-Martin and chocolate agar? When would you use Thayer-Martin rather than chocolate agar?List all the possible reasons why the bacteria in broth suspension that were spread and pour plated are poorly grown.You inoculate a species of bacteria on a MacConkey agar. It normally shows positive for growth and ability to breakdown lactose. What other method would you use to confirm the result of the MacConkey agar, and what result would you expect?
- Suppose your Professor handed you a test tube with 2.0mL of E.coli broth culture and told you to make a 10-1 dilution of the ENTIRE culture. Explain how you would do this.Which ONE of the following best describes the abbreviation ‘MIC’? Select one: A. The lowest concentration at which no microbial growth is detected B. The lowest concentration that indicates whether an antibiotic would be effective or not C. The lowest concentration which is not toxic to the host D. The lowest concentration which is least effective against the organism E. The lowest concentration which kills the organismThe following results were obtained from a disk diffusion test for a strain of Escherichia coli. Chemotherapeutic Zone of inhibition (mm) A 7 B 10 C 18 D 3 Which drug above (A, B, C, or D) is most effective against this E. coli strain? ***Which drug above (A, B, C, or D) is most effective against Staphylococcus aureus? Explain your answer.
