Describe how you would prepare 500mL Edward’s buffer when you have 1M Tris-CL (pH 7.5), 1M NaCl, 50mM EDTA, and 10% SDS in the lab. the final concentration in Edward’s buffer: 200mM Tris-Cl, pH 7.5: lysis buffer, regulates pH and osmolality 250mM NaCl: removes protein bound to DNA 25mM EDTA (Ethylenediaminetetraacetic acid): Chelating agent that depletes metal ions 0.5% SDS (sodium dodecyl sulfate): surfactant commonly found in cleaning products.  In this lab it is used to lyse cells.

Question

Describe how you would prepare 500mL Edward’s buffer when you have 1M Tris-CL (pH 7.5), 1M NaCl, 50mM EDTA, and 10% SDS in the lab.

  • the final concentration in Edward’s buffer:
  • 200mM Tris-Cl, pH 7.5: lysis buffer, regulates pH and osmolality
  • 250mM NaCl: removes protein bound to DNA
  • 25mM EDTA (Ethylenediaminetetraacetic acid): Chelating agent that depletes metal ions
  • 0.5% SDS (sodium dodecyl sulfate): surfactant commonly found in cleaning products.  In this lab it is used to lyse cells.

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