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Asked Feb 15, 2020
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Describe the techniques used to identify macromolecules.

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Expert Answer

Step 1

Macromolecules are polymers that are composed of the sequence of smaller molecules called monomers. The formation of macromolecule is an energy-requiring process called "polymerization" that releases water as a byproduct. Each process varies based on the type of macromolecule being formed.

The four major biological macromolecules are carbohydrates, lipids, proteins, and nucleic acids.

Step 2

There are various analytical methods in biochemistry used to separate, identify and qualify macromolecules. Some of them are, chromatography, electrophoresis, spectrophotometer, centrifugation, and Enzyme-linked immunosorbent assay.

Step 3

Chromatography is an analytical technique that allows the identification, separation, and determination of macromolecules in the mixture. There are different types of chromatography such as paper chromatography, affinity chrom...

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