Describe the whole-genome shotgun approach

Q: Describe at least five (05) different types of commercially available vectors for cloning with size…

A: Cloning vectors are small DNA parts that are stably maintained in an organism. The foreign fragment…

Q: Enlist the goals of Human genome project.

A: The Human Genome Project (HGP) is an international project that included thirteen years work of…

Q: Write down all possible outcomes for template ATGCCTAAGTTTCCCTAT sequencing

A: Deoxyribonucleic acid (DNA) is the macromolecule that stores genetic information. It is present in…

Q: Explain the purpose of genome annotation.

A: A genome is all genetic material of a living being. It comprises of DNA (deoxyribonucleic…

Q: Name any two diseases for which transgenic mice are used as model organisms.

A: The transgenic mouse is obtained by the introduction of foreign DNA into the genome of the mouse. It…

Q: Compare the differences in the techniques used in whole genome shotgun sequencing and next…

A: The process of determining the exact order of nucleotides in a gene, a cluster of genes, a…

Q: Describe the benefits of using whole genome sequencing for diagnosis.

A: The whole genome shotgun method involves cloning, followed by accurate computer sequencing of the…

Q: Define about stoneage genomics ?

A: The discovery of ancient genomics such as stoneage genomics has revolutionized our understanding of…

Q: Outline different methods of DNA sequencing.

A: The sequence of a DNA (deoxyribonucleic acid) molecule is the order in which deoxyribonucleotides,…

Q: Sanger sequencing Write a precise and accurate differential report on the above sequencing…

A: DNA sequencing is the process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a…

Q: list and explain two strategic between the shotgun sequences/ assemble strategy for whole genome…

A: Genomic Sequencing is a process where whole genome of an organism is sequenced. With the help of…

Q: State and describe the benefits of using whole-genome sequencing for diagnosis.

A: The whole genome shotgun method involves cloning, followed by accurate computer sequencing of the…

Q: compare and contrast different approaches for assessing genome assemblies using assembly…

A: Genes are hereditary functional units that are made up of DNA (deoxyribonucleic acid). The genome is…

Q: explain why gene mapping is important and how this technique can be used in disease investigation?

A: The Human Genome Project aimed at developing tools to identify new genes and their function. Genetic…

Q: Compare tblastx and tblastn software packages of genome sequence analysis.

A: BLAST stands for Basic Local Alignment Search Tool •What makes BLAST so popular??- Good balance of…

Q: What are the importance of dosage forms? Explain your answer

A: The method by which pharmaceutical drug molecules are delivered within the body to the site of the…

Q: Define about limitation of most recombinant DNA approaches ?

A: Recombinant DNA approaches are the process where DNA of multiple sources is taken and formed into a…

Q: Write precise and accurate differential report on the sequencing techniques

A: Genomics is the study of an organism's entire genome, which contains genetic elements. To sequence,…

Q: Discuss the Genetically Modified Organism (Benefits and Risks)

A: GMOs are organisms whose genetic material have been altered through the application of genetic…

Q: To discuss: The value of genome projects.

A: Recombinant DNA technology (rDNA technology) is extremely useful for analyzing a species' entire…

Q: process of overlap assembly in whole genome sequencing

A: Genome sequencing Genome sequencing or gene sequencing is a way of arranging nucleotides the n the…

Q: Write an accurate and precise differential report on the next generation sequencing technique

A: The biological sciences have been transformed by next-generation sequencing (NGS). NGS helps…

Q: Describe the shotgun method for determining thecomplete genome sequence of an organism.

A: Biotechnology is the branch of science that explain the origin of Genetically modified organisms

Q: Give (3) three applications or uses of Human Genome Project and briefly explain each..

A: The Human Genome Project (HGP) was an international scientific research project that aimed to…

Q: write a short note on different available methods for DNA SEQUENCING? proper explanation and diagram

A: The process of determining the order of nucleotides (basically, the order of four nitrogenous bases-…

Q: Contrast and compare the advantages and disadvantages of the Sanger method with next-generation…

A: Sanger and NGS are two sequencing methods for analyzing the sequences of DNA. The benefits of Sanger…

Q: Describe the ethical issues encompassing germ line gene therapy in 150 words.

A: Introduction Germline gene therapy:- In this therapy DNA is inserted into the reproductive cells…

Q: Describe the uses of BLAST searches in comparativegenomics.

A: BLAST (basic local alignment search tool) is a tool in bioinformatics that compares the different…

Q: Briefly explain Polymerase Chain Reaction (PCR) applications in clinical diagnosis.

A: Polymerase chain reaction (PCR) is a molecular biology-based laboratory method for multiplying a…

Q: What are the purposes of Suppositories dosage form

A: Suppository It is administered to produce either systemic or local effect, in solid dosage form.…

Q: Explain the rationale and achievable goals behind using a genetic screen(like Heidelberg screen).…

A: Genetic screening such as Heidelberg screening is also called mutagenesis screening. This technique…

Q: What is the one main advantage of the whole-genome shotgun strategy over the hierarchical approach?

A: Genome sequencing is a vital process that helps in biological and medical analysis.

Q: Define hybridization (sanger sequencing)

A: Sanger sequencing is a method which is used to determine the sequence of nucleotides in DNA. This…

Q: Explain about personal genomics approach ?

A: Finding the nucleic acid sequences, or the arrangement of nucleotides in DNA, is the procedure of…

Q: Discuss the principle ,uses, advantages and disadvantages of shotgun sequencing.

A: DNA sequencing are very essential to delineate the exact order and arrangement of nucleotides within…

Q: Define about whole-genome sequencing (WGS) ?

A: The study of genomes' design, functioning, development, sequencing, and modification is the emphasis…

Q: Describe the differences between the hierarchical andshotgun strategies for genome sequencing.

A: There are two approaches exist in sequencing of huge genomes. These are as follows: The…

Q: Define about Human Genome Project-Write (HGP-Write) ?

A: An international, collaborative research program called the Human Genome Project (HGP) aimed to map…

Q: Compare the knowledge obtained from geneticdissection and from genome sequencing.

A: The term genome refers to the entire genetic material of an organism. Human genomes have about 3…

Q: Describe the whole-genome shotgun strategy

A: BASIC INFORMATION SHOTGUN SEQUENCING In this random DNA strands are sequenced. It is named so…

Q: Explain briefly : (a) PCR

A: PCR stands for Polymerase Chain Reaction. PCR is used to make multiple copies of a gene.

Q: Detail explanation on next generation sequencing

A: Next-generation sequencing is a combination of methods that have produced much higher throughput…

Q: Compare and contrast the assembly of genomes using Sanger and next-generation sequencing (NGS)…

A: Genome sequencing: genome means total gene content present in the organism and sequence means to…

Q: Select a gene of interest and do BLAST search, and describe results in details

A: BLAST is a program family, that allows to enter a query sequence and compare it to DNA or protein…

Q: Define about whole-genome sequencing ?

A: There may be millions of genes present in the organisms. It is not possible to sequencing all types…

Q: Give a schematic diagram of how we can Treatment Down's syndrome  by using gene therapy? Please…

A: Gene therapy : It involves altering the genes inside your body cells in an effort to treat or stop…

Q: describe next generation sequencing technique

A: The biological sciences have been transformed by next-generation sequencing, a massively parallel…

Q: Explain the example of predominant method for sequencing genomes ?

A: The study of genomes' design, functioning, development, sequencing, and modification is the emphasis…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.