Determine what percentage of the culture was living (viable) and what percentage was dead (mortality). PlatesPlate DilutionVolume platedNo.of coloniesAvg NoConcentration of diluted sample Cd(cells/mL)Concentration of original sample Cu(cells/mL)110^-310ulR1=130,R2= 110,R3=210150150mL1.50*10^6Volume of cells(mL)Volume of diluent(mL)Total dilution(D)Hemocytometer count Avg cells in 1 mm^2 areaConcentration of diluted sample Cd(cells/mL)Concentration of original sample Cu(cells/mL)4.30.50.1grid 1= 171 , grid2 = 1851781.78*10^51.78*10^6

The word "CFU" in microbiology refers to a colony-forming unit, which is used to estimate how many…

Answered: Determine what percentage of the…

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Biology

Determine what percentage of the culture was living (viable) and what percentage was dead (mortality).

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.8: Fecal Occult Blood Test

Problem 1CS

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Determine what percentage of the culture was living (viable) and what percentage was dead (mortality).

Plates	Plate Dilution	Volume plated	No.of colonies	Avg No	Concentration of diluted sample Cd(cells/mL)	Concentration of original sample Cu(cells/mL)
1	10^-3	10ul	R1=130,R2= 110,R3=210	150	150mL	1.50*10^6

Volume of cells(mL)	Volume of diluent(mL)	Total dilution(D)	Hemocytometer count	Avg cells in 1 mm^2 area	Concentration of diluted sample Cd(cells/mL)	Concentration of original sample Cu(cells/mL)
4.3	0.5	0.1	grid 1= 171 , grid2 = 185	178	1.78*10^5	1.78*10^6

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