Determine whether traditional polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR), or quantitative PCR (qPCR) should be used for each application PCR RT-PCR ДРCR cloning DNA sequences detecting differential exon use genotyping cloning MRNA sequences verifying microarray data quantification of gene expression Answer Bank о

Determine whether traditional polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR), or quantitative PCR (qPCR) should be used for each application PCR RT-PCR ДРCR cloning DNA sequences detecting differential exon use genotyping cloning MRNA sequences verifying microarray data quantification of gene expression Answer Bank о

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter14: Biotechnology And Society

Section: Chapter Questions

Problem 8QP: DNA Profiles as Tools for Identification STRs are: a. used for DNA profiles b. repeated sequences...

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As you learned in this chapter,restriction enzymes are sophisticated“scissors” that geneticistsand molecular biologists routinelyuse to cut DNA for recombinant DNAexperiments. A wide variety of onlinetools assist scientists working with restrictionenzymes and manipulating recombinantDNA for different applications. Herewe explore Webcutter and Primer3, twosites that make recombinant DNA experimentsmuch easier.Exercise I – Creating a RestrictionMap in WebcutterSuppose you had cloned and sequenceda gene and you wanted to design a probeapproximately 600 bp long that could beused to analyze expression of this gene indifferent human tissues by Northern blotanalysis. Internet sites such as Webcuttermake it relatively easy to design experimentsfor manipulating recombinant DNA.In this exercise, you will use Webcutter tocreate a restriction map of human DNAwith the enzymes EcoRI, BamHI, and PstI.1. Access Webcutter at http://www.firstmarket.com/cutter/cut2.html.Go to the Study Area for…
Write and briefly define the steps sequentially to clone the gene from the genomic DNA of the organism by PCR, into pGEMT vector and check for the presence of insert.. Important- Provide illustrations, examples, and diagrams wherever applicable.
DNA extraction, PCR, gel electrophoresis, and DNA sequencing of PCR products. Give a description of these methods and the materials required to perform each of these molecular techniques
For each of the restriction enzymes listed below:(i) Approximately how many restriction fragmentswould result from digestion of the human genome(3 × 109bases) with the enzyme? (ii) Estimate theaverage size of the pieces of the human genomeproduced by digestion with the enzyme. (iii) Statewhether the fragments of human DNA produced bydigestion with the given restriction enzyme wouldhave sticky ends with a 5′ overhang, sticky endswith a 3′ overhang, or blunt ends. (iv) If the enzymeproduces sticky ends, would all the overhangs on allthe ends produced on all fragments of the humangenome with that enzyme be identical, or not? (Therecognition sequence on one strand for each enzymeis given in parentheses, with the 5′ end written atthe left. N means any of the four nucleotides; R is any purine—that is, A or G; and Y is any pyrimidine—that is, C or T. ^ marks the site of cleavage.)a. Sau3A (^GATC)b. BamHI (G^GATCC)c. HpaII (C^CGG)d. SphI (GCATG^C)e. NaeI (GCC^GGC)f. BanI (G^GYRCC)g. BstYI…
DNA Profiles as Tools for Identification STRs are: a. used for DNA profiles b. repeated sequences present in the human genome c. highly variable in copy number d. all of these e. none of these
Positional cloning refers to:a. using a selection procedure to clone a cDNAb. cloning a portion of a gene using PCRc. isolating a gene by PCR using primers from another speciesd. isolating a gene from a specific tissue in which it is being expressede. mapping a gene to a chromosomal region and then identifying and cloning a genomic copy of the gene from the region
You are fresh recruits to a molecular biology laboratory, and your new boss has tasked you tostudy mutations in NRAS, a gene that she suspects is involved in cancer pathogenesis. Task A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from cDNA extracted from differentsamples through PCR. You will need to run a total of 7 PCRs: 3 normal samples, 3 canceroussamples, and 1 negative control. To make things easier in the lab, when running multiplereactions, the components are prepared not individually, but as a master mix—all thecomponents for multiple reactions are prepared in bulk, except for the template DNA, which isadded separately once the master mix has been distributed into individual tubes. The table below lists the different components for PCR, the available stock concentrations of thesecomponents, and the needed working concentrations for the PCR itself. Complete the table bysupplying the needed volumes of each component for a single…
Both microarray and RNA-sequencing can study the transcriptomes. Compare microarray and RNA sequencing technique in analyzing gene with high, medium and low copy number of genes.
Describe PCR condition to amplify the sequence described Q.No.1 (Select a gene of interest and do BLAST search, and describe results in details.) using primers developed in Q. No. 2 (Design the primers from the sequence used in Q.No.1 and describe its properties in detain showing suitability of primer.)
Explain why DNA ladders are usually included during gel electrophoresis. One aspect of PCR that can be modified is the annealing temperature. In general, higher annealing temperatures show more specificity towards a single template, whereas lower annealing temperatures show less specificity and may bind to multiple regions throughout the genome. Discuss how using an annealing temperature that is too high or too low might influence the results of a PCR assay (and gel electrophoresis results) such as the one used in this study.
18) Ramachandran plot (how to read it) 19 )Rotations around bonds in a peptide sequence 20) DNA and RNA based techniques 21) Difference between DNA and RNA 22) PCR 23) RT-PCR 24) DNA synthesis and sequencing 25) Restriction enzymes 26) Techniques to determine gene function 27) SDS-PAGE 28) Reading DNA, RNA and protein sequences 29) Cloning vectors 30) Stereoisomerism and amino acids 31) Protein and nucleotide based electrophoresis and blotting 32) Centrifugation
Select all that apply: Which of these components must be added to a PCR reaction for it to produce a product?O DNA PrimersO Buffers- dNTPsO RNA PrimersO NTPSO PrimaseO Template DNAO Taq DNA Polymerase