Discuss the importance of adding radioactively labeled molecules during 16S ribosomal RNA sequencing process of a bacterial genome.

Q: scribe the process of cloning a DNA fragment into the EcoR1and AluI sites of the vector pUC18

A: pUC multiple cloning site (MCS) contains lacZ gene which encodes for beta galactosidase (b-gal)…

Q: Starting with a sample of RNA that contains the mRNA for theβ-globin gene, explain how you could…

A: DNA is the genetic material that carries genetic information in the form of coded nucleotide…

Q: Explain your experimental approach if you must correct the genetic defect phenylketonuria, which is…

A: Phenylketonuria (commonly known as PKU) is an inherited disorder that increases the levels of a…

Q: Discuss how scientists employ a bacteriophagesite-specific recombination system to generateknockin…

A: Knockin mouse is a method in gene recombination in which an exogenous gene of interest is inserted…

Q: ACAACCCCAAGCCTTC

A: Answer : PROBE - DNA probes are the short stretches of single stranded DNA used to detect the…

Q: Explain the origin of the signal that is used in the nucleic acid sequencing method known as…

A: Ion torrent technology is a modern genome sequencing method that converts chemically encoded…

Q: Discuss how recombinant DNA technology has improved the production of the following: Restriction…

A: There are few important points about recombinant DNA technology . Which are as follows . It is also…

Q: The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy.…

A: Enzymes  Enzymes are the protein that use as catalysts in metabolic and catabolic reaction to fasten…

Q: In CRISPR genome editing technology, when the Cas9 protein cleaves the target DNA sequence, what…

A: The microorganism depends on multiple defense strategies that support them to resist viral attack.…

Q: Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing,…

A: Hi! Thank you for your question. We are answering your question on the basic knowledge of the…

Q: Discuss the use of gel electrophoresis for the separation of macromolecules (DNA, RNA and protein)…

A: Gel Electrophoresis It is a technique which is used to separate the fragment of macromolecules like…

Q: outline HOW to clone and identify a new gene!

A: Gene clonning is technique of genetic engineering where a gene of intrest is isolated from its DNA…

Q: What are the vectors used in rDNA technology? briefly explain each

A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…

Q: Select the definition of a microsatellite. short pieces of DNA surrounding the periphery of the…

A: Answer

Q: Name any two cloning vectors. Describe the features required to facilitate cloning into a vector.

A: Cloning is a process that allows scientists to create perfect genetic duplicates of living…

Q: process of overlap assembly in whole genome sequencing

A: Genome sequencing Genome sequencing or gene sequencing is a way of arranging nucleotides the n the…

Q: Identify the mRNA sequence that encodes the protein Design primers that will allow them to amplify…

A: Deoxyribonucleic acid(DNA) is a molecule comprised of two polynucleotide chains coiled around each…

Q: Please list all possible products in a sequencing reaction using ddGTP as a terminator based on the…

A: 1.As dd GTP is used, every time it encounters C base it will terminate. 2.When C is encountered with…

Q: Explain why a selectable marker is frequently included in a cloning vector.

A: A cloning vector is a short bit of DNA that may be kept in an organism for a long time and into…

Q: Technique whereby inserting DNA into a clone is accomplished using two different restriction enzymes

A: Directional cloning is the technique which is accomplished by cleaving the plasmid vector with two…

Q: Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and…

A: Hello. Since your question has multiple sub-parts, we will solve first three sub-parts for you. If…

Q: What type of enzymes are used to “cut” desired DNA sequences for use in recombinant gene technology…

A: Note: As Per Guidelines, We Can Answer One Question At A Time. Ask Again To get rest answers.…

Q: What are the differences between somatic and germline gene editing? Is there any scientific example…

A: Gene therapy is a widely used technique to treat genetic disorders. There are two types of gene…

Q: Compare sequencing method 1 Illumina and sequencing method PacBio for: a. Read length. What are the…

A: Illumina sequencing methods are also called short read methods as they read shorter sequences in a…

Q: Explain and list down the protocols in doing or preparation of materials for polymerase chain…

A: Polymerase chain reaction (PCR) It is an in vitro technique that takes a specific sequence of DNA…

Q: Identify which mutagen is described by the following statement. Inserts between adjacent bases in…

A: Certain chemical mutagen can insert between adjacent base pairs of DNA molecule e.g., proflavine…

Q: has been assembled by researchers and transplanted into a donor bacterial strain to study never…

A: A- recombinant plasmid  B- developmental genetics

Q: Please answer ASAP RNA sequencing (RNA-Seq) of one sample in one run of a massively parallel…

A: Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers…

Q: Refer to the figure. What method would you use if you wanted to determine the sequence of the cDNA…

A: cDNA is complementary DNA. This DNA is artificially synthesized from the mRNA template. In genetic…

Q: Consider the following human genetic diseases: hemophilia, Down syndrome, cystic fibrosis, and brain…

A: CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats) Cas genome editing technology…

Q: Discuss the main similarities and differences in the nucleic acid sequencing methods known as…

A: DNA sequencing is the process of determining the sequence of nucleic acids, which are the basic…

Q: Describe the features of a typical CRISPR locus in a bacterial cell.

A: CRISPR (short for clustered regularly interspaced short palindromic repeat) acts as the immune…

Q: Use this information to match the list of probes to the 3 samples below: You have isolated several…

A: Southern blotting and Northern blotting are used to isolate and identify nucleic acid present in the…

Q: Define the following terms:a. nonreplicative transpositionb. replicative transpositionc. composite…

A: Transposons are DNA (deoxyribonucleic acid) segments that have the internal property of movement…

Q: Consider the reagents required to perform CRISPR genome editing, Which molecule specifies the a new…

A: CRISPR genome editing is a genetic engineering technology in which the genomes of individual are…

Q: Describe how to select recombinant clones if a foreign DNA is inserted into the polylinker site of…

A: Introuction- pUC18 is a 2686 bps long plasmid vector which contains pMB1 as origin of replication…

Q: the strategies commonly used to detect for a named cloned gene in E.coli

A: Gene cloning, otherwise known as molecular cloning, alludes to the manner toward confining a polymer…

Q: The best molecular technique to quantify the gene transcripts is (write in fu!!).

A: The Central Dogma concept states that "DNA makes RNA and RNA makes protein". This concept is…

Q: Briefly explain why RNA-seq gives more information about the transcriptome than does microarray…

A: Messenger (m-RNA) ribonucleic acid is known to be transcribed from the DNA molecule, which is the…

Q: CRISPR RNA (crRNA) in a bacterial cell: is a snippet of genetic material from a virus stored in a…

A: The CRISPR/Cas9 or clustered regularly interspaced short palindromic repeats and CRISPR-associated…

Q: Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Describe the process of cloning a DNA fragment into the EcoRI site of the Charon 4 vector. by…

A: Cloning is a procedure to make several identical copies of a DNA (Deoxyribonucleic acid). Charon 4…

Q: To achieve a 10x depth coverage for a genome of length 10 Mbp, how many reads of 100 bp are…

A: A Coverage is multiplier based on  total size of the genome. Formula =  Coverage = (read length) x…

Q: Review DNA sequencing and cloning tools. Match term and its description. a cloning vector that…

A: A cloning vector that contain a highly active bacterial promoter is called the expression vector .…

Q: What size bands would you expect to see on the Southern blots using probe 1 and probe 2 for an…

A: Introduction The term Sickle cell disease defines the condition where due to a mutation, the blood…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.