Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.8% agarose gel, where the percentage is determined as mass/vol. How many grams of agarose must she add to 125 mL of buffer in order to arrive at the correct percentage? (answer to 2 decimals) **Assume the dissolution of agarose will not alter the final volume of the solution** Polymerase Chain Reaction (PCR) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme from Thermophilus aquaticus to amplify DNA extracted from any sample. In order for the reaction to work, researchers must transfer microliters of sample to the reaction vessel for PCR. If a researcher has a DNA sample with a concentration of 0.029 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 4.6 nanograms per microliter? Report your answer to two decimal places using normal decimal notation (e.g. 0.5, not 5.0e-1)

Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.8% agarose gel, where the percentage is determined as mass/vol. How many grams of agarose must she add to 125 mL of buffer in order to arrive at the correct percentage? (answer to 2 decimals) Assume the dissolution of agarose will not alter the final volume of the solution Polymerase Chain Reaction (PCR) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme from Thermophilus aquaticus to amplify DNA extracted from any sample. In order for the reaction to work, researchers must transfer microliters of sample to the reaction vessel for PCR. If a researcher has a DNA sample with a concentration of 0.029 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 4.6 nanograms per microliter? Report your answer to two decimal places using normal decimal notation (e.g. 0.5, not 5.0e-1)

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter31: Immunochemistry

Section: Chapter Questions

Problem 31.20P: Chemical Connections 20D states that the antigen in the red blood cells of a person with B-type...

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Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.2% agarose gel, where the percentage is determined as mass/vol. How many grams of agarose must she add to 200 mL of buffer in order to arrive at the correct percentage?
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Q23: Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.1% agarose gel, where the percentage is determined as mass/vol. How many grams of agarose must she add to 413 mL of buffer in order to arrive at the correct percentage? (answer to 2 decimals) **Assume the dissolution of agarose will not alter the final volume of the solution**