As shown, five DnaA boxes are found within the origin of replication in E. coli. Take a look at these five sequences carefully.A. Are the sequences of the five DnaA boxes very similar to each other? (Hint: Remember that DNA is double-stranded; think about these sequences in the forward and reverse directions.)B. What is the most common sequence for a DnaA box? In other words, what is the most common base in the first position, second position, and so on until the ninth position? The most common sequence is called the consensus sequence.C. The E. coli chromosome is about 4.6 million bp long. Based on random chance, is it likely that the consensus sequence for a DnaA box occurs elsewhere in the E. coli chromosome? If so, why aren’t there multiple origins of replication in E. coli? E. collchromosomeoricAT-rich reglon5'-GGATCCTGGGTATTAAAAAGAAGATCTATITATITAGAGATCTGTTCTATCCTAGGACCCATAATTTITCITCTAGATAAATAAATCTCTAGACAAGATA50DnaA boxTсTGATCTCTTАТTAGGATCGCACтсссстстGGATAАСAAGGATCGGCтẠCACTAGAGAATAATCCTAGCGTGACGGGACACCTATIGTTCCTAGCCGA10051DnaA boxTTTAAGATCAACAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATAAATTCTAGT TGTTGGACCTTTCCTAGTAATTGACACTTACTAGCCACTA101150DnaA boxсстGGACCGTATАAGCTGGGATCAGAATGAGGGTTATАCACAGCTCAAAAGGACCTGGCATATICGACCCTAGTCTTACTCCCAATATGIGICGAGTIIT200151ACTGAACAACGGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTȚGACTTGTTGCCAACAAGAAACC TATIGATGGCCAACTAGGTICGAAGGA201DnaA box250DnaA boxGACAGAGTTATCCACAGTAGATCGC -3'стстстCAATAGGTGTCATCTAGCG251275

E.coli. is a key model organism and has been used in so many research studies involving molecular…

Answered: E. coll chromosome oric AT-rich reglon…

1st Edition

ISBN:9781938168130

Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble

Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble

Chapter18: The Cardiovascular System: Blood

Section: Chapter Questions

Problem 28CTQ: Why would it be incorrect to refer to the formed elements as cells?

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Question

As shown, five DnaA boxes are found within the origin of replication in E. coli. Take a look at these five sequences carefully.

A. Are the sequences of the five DnaA boxes very similar to each other? (Hint: Remember that DNA is double-stranded; think about these sequences in the forward and reverse directions.)

B. What is the most common sequence for a DnaA box? In other words, what is the most common base in the first position, second position, and so on until the ninth position? The most common sequence is called the consensus sequence.

C. The E. coli chromosome is about 4.6 million bp long. Based on random chance, is it likely that the consensus sequence for a DnaA box occurs elsewhere in the E. coli chromosome? If so, why aren’t there multiple origins of replication in E. coli?

E. coll
chromosome
oric
AT-rich reglon
5'-GGATCCTGGGTATTAAAAAGAAGATCTATITATITAGAGATCTGTTCTAT
CCTAGGACCCATAATTTITCITCTAGATAAATAAATCTCTAGACAAGATA
50
DnaA box
TсTGATCTCTTАТTAGGATCGCACтсссстстGGATAАСAAGGATCGGCт
ẠCACTAGAGAATAATCCTAGCGTGACGGGACACCTATIGTTCCTAGCCGA
100
51
DnaA box
TTTAAGATCAACAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGAT
AAATTCTAGT TGTTGGACCTTTCCTAGTAATTGACACTTACTAGCCACTA
101
150
DnaA box
сстGGACCGTATАAGCTGGGATCAGAATGAGGGTTATАCACAGCTCAAAA
GGACCTGGCATATICGACCCTAGTCTTACTCCCAATATGIGICGAGTIIT
200
151
ACTGAACAACGGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCT
ȚGACTTGTTGCCAACAAGAAACC TATIGATGGCCAACTAGGTICGAAGGA
201
DnaA box
250
DnaA box
GACAGAGTTATCCACAGTAGATCGC -3'
стстстCAATAGGTGTCATCTAGCG
251
275

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