Examine the limitations of pedigree analysis inproviding the information needed for positional cloning.

Q: How can the concept ofrecombination frequency beused in genetic mapping?

A: Recombination is a process of exchange of genetic material between two different organisms.…

Q: What are genetic mapping studies?

A: Gene mapping is used to map the relative positions of genes on a chromosome using a cross over…

Q: List at least two ways in which computerized analysis oftranscriptomes aids genetic research.

A: Genetic research refers to the study of human deoxyribonucleic acid (DNA) to order to know the genes…

Q: Describe at least five (05) different types of commercially available vectors for cloning with size…

A: Cloning vectors are small DNA parts that are stably maintained in an organism. The foreign fragment…

Q: List the three features of a cloning vector and why these elements are needed

A: Cloning vectors act as a vehicle to transfer desired foreign genetic material into a cell. All these…

Q: Using illustrations describe the structure of a typical cloning vector and discuss the functions of…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: Comment on the utility of variability in number of tamndem repeats during DNA fingerprintingds?

A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…

Q: Outline the main steps in cloning a gene, including how this geneis used.

A: Gene cloning is also a molecular technique used in molecular biology to amplify the DNA segment by…

Q: Explain the disadvantages of searching for homologous genes and proteins.

A: Homologous genes or protein are searched by using various bioinformatical tools like BLAST, FASTA,…

Q: Describe step 1: hybridization of template and primer in automated sanger sequencing

A: Introduction  Sanger sequencing is also known as the “chain termination method”. It is a method for…

Q: What provides a convenient bridge between the low resolution of a karyotype and the ultra-high…

A: Introduction  Cytology refers to the study of cell such as cell morphology, physiology etc. As we…

Q: Explain the basic techniques used to manipulate genetic material

A: Genetic Material:         A cell or an organism comprises information carrier materials found in the…

Q: What is the relevance of the sample size to the choice of test organisms for genetic studies?

A: The sample size is the number of organisms chosen for a study or experiment. It can be large or…

Q: Define the positional cloning (sometimes called reverse genetics). ?

A: Reverse genetics is a molecular genetics technique that allows researchers to deduce gene function…

Q: Design forward and reverse primers to amplify this ENTIRE locus by PCR. Design each primer to be 10…

A: The forward and reverse primers are both important components used in PCR for amplifying a target…

Q: Briefly describe the steps or methods for studying genes.  Start with targeting a gene region and…

A: Gel electrophoresis is a strategy for investigating DNA dependent on its size (base-pair length). As…

Q: Which of the following technologies can best differentiate between a diploid wild banana and a…

A: Edible banana have either 22 , 33 or 44 chromosomes that represent diploid , triploid and…

Q: Prepare an X µL PCR reaction mixture and set thermal conditions for gene amplification?

A: PCR is a strong amplification technique that can produce a large amount of a particular DNA segment…

Q: How is DNA microarray technology used to determine the differences in gene expression?

A: A Deoxyribonucleic acid (DNA) microarray is a collection of microscopic DNA spots attached to a…

Q: Explain why a selectable marker is frequently included in a cloning vector.

A: A cloning vector is a short bit of DNA that may be kept in an organism for a long time and into…

Q: describe how DNA shuffling and molecular breeding are used to generate hybrid genes and the…

A: DNA shuffling or molecular breeding is an in-vitro molecular method, where mutant genes are created…

Q: Explain the concept of Making a Transgenic Animal: The Basics ?

A: The production of transgenic domesticated animals has the potential to significantly improve human…

Q: How to find unknown gene and design primers present using whole genome sequence?

A: Step 1 Whole genome sequencing is the process that regulates the complete deoxyribonucleic acid…

Q: Discuss the advantages of next-generation sequencing technologies.

A: Next generation sequencing technique is also known as second generation sequencing where the DNA…

Q: Discuss the role (presence and absence) of a selectable marker (selection) in cloning a recombinant…

A: Recombinant DNA molecule is defined the molecule in which an isolated DNA fragment or gene of…

Q: Give the different uses of proteomics in the field of genetics.

A: The nucleus of the cell contains DNA on which coding sequences known as genes are located. The genes…

Q: You may have heard of Dolly, the cloned sheep grown from an embryo created in a laboratory. But in…

A: Dolly is the sheep, which is considered as the first genetically modified (GMO) organism, which was…

Q: Explain the experimental advantage of genetic mapping?

A: Gene mapping was discovered by Alfred H. Sturtevant. He discovered this in order to identify the…

Q: Define hybridization (sanger sequencing)

A: Sanger sequencing is a method which is used to determine the sequence of nucleotides in DNA. This…

Q: Give An example of positional cloning?

A: Cloning is a technique in which a desired gene segment is combined with another DNA molecule of so…

Q: Consider and interpret the results of agarose gel electrophoresis and spectrophotometer for the…

A: Answer. Electrophoresis of nucleic acids: Gel electrophoresis is the process by which scientists can…

Q: Describe why genetic mapping is useful?

A: Gene is a specific nucleotide sequence in RNA or DNA. It is generally located on a chromosome. In…

Q: Mention any two applications of DNA fingerprinting.

A: Deoxyribonucleic acid (DNA) is a molecule composed of two polynucleotide chains. It coils around…

Q: Describe how a DNA microarray is constructed and howto genotype millions of loci on this microarray

A: A DNA microarray is also commonly called as DNA chip or Biochip. A microarray is a collection of…

Q: what are Several Limitations of positional cloning?

A: Positional cloning is one of the laboratory technique of gene identification. In this method, gene…

Q: Explain how mutations were used to verify the geneticcode.

A: Genetic code is defined as a set of rules that decodes the information from DNA into a protein. A…

Q: Briefly explain how a gene can be isolated through positional cloning.

A: A positional cloning is a technique used in laboratory for locating the position of a gene…

Q: Explain the rationale behind the use of the “bag of worms” phenotype as a way to identify…

A: A mutation refers to an alteration in the genome or genetic material of a living organism’s cells or…

Q: explain Mutations: Primary Tools of Genetic Analysis

A: DNA is the main constituent of the chromosome. It contains all information about protein that forms…

Q: Describe the important features of cloning vectors. Explain the purpose of selectable markers in…

A: Cloning vector refers to small piece of deoxy ribonucleic acid (DNA), which are used to propogate…

Q: Explain the procedures for Random Amplified Polymorphic DNA

A: Random Amplified Polymeric DNA (RAPD) are DNA fragments amplified by PCR using short synthetic…

Q: Differentiate between a PCR cycle and step, and define the function of each of the three steps used…

A: Polymerase chain reaction or PCR is a technique applied on DNA. DNA is a polymer of multiple…

Q: Explain the different vectors are available for cloning ?

A: Cloning vectors are the DNA molecules in which the desired DNA segments are inserted.