Explain Gene therapy with viral vectors?

Q: Define transfection.

A: A mutation is a change or alteration in the nucleotide sequence of the genome of any organism.…

Q: Compared with cells, what is unusual about viral genomes?

A: A virus is a submicroscopic infectious particle that replicates only inside the living cells of an…

Q: Why are retroviruses important?

A: A virus is composed not of DNA but of RNA. Retroviruses have an enzyme, called reverse…

Q: What are retroviruses?

A: Viruses are simpler in structure and composition. They are made up of nucleic acids and proteins.…

Q: Identify the Properties of Viruses?

A: Viruses are the smallest infectious agents that contain single or double-stranded nucleic acid…

Q: Which of the following viruses have been used as vectors for gene delivery?

A: Gene delivery is a process of introducing the foreign genetic materials which are DNA and RNA into…

Q: Explain how to determine the number of plaque-forming units in a virus sample?

A: There are viruses that infect the bacteria, called bacteriophages. The presence of such viruses can…

Q: How do 3D protein modeling tools help in the development of drugs or vaccine?

A: The 3D structures of the desired target proteins, drugs and epitopes can be predicted and modeled…

Q: What are the considerations for choice of a vector in gene therapy?

A: HIV consists of single-stranded RNA in the replication form that has been transcribed into…

Q: What do you think is the reason why this virus undergoes mutation?

A: A virus is a submicroscopic infectious agent that replicates only within an organism's live cells.…

Q: Define the Gene-targeting methods ?

A: Gene targeting method can be defined as the genetic engineering technique which uses the…

Q: Can phage kill virus?

A: Viruses are microscopic agents that can replicate only inside host cells. They can infect all types…

Q: Why is earlier to target a single mutant versus multiple mutants in the virus

A: Mutations are the changes in the DNA sequence of an organism which may or may not affect the…

Q: Can antibiotic kill virus?

A: Antibiotics can't kill  virus. Antibiotics are medicines which kill bacterial infection.

Q: Explain Oncogenes and retroviruses?

A: Virus is a small microscopic agent which is an infectious agent that can replicate only inside the…

Q: What are the two type of viral vector involved in gene therapy trials?

A: Viral vectors are the engineered tools which are used by molecular biologists to transfer genetic…

Q: What is an example of a Lysogenic virus?

A: Bacteriphage have two types of cycle. Lytic cycle- It involves replication of virus using host cell…

Q: explain  Resistance plasmids

A: Plasmid is a genetic structure in a cell that can replicate independently of the chromosomes,…

Q: What is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

A: Gene therapy is a method that allows the correction of the defective gene. In gene therapy, it is…

Q: How does phage therapy work?

A: Phages are viruses that use bacterial as hosts. Some of the phages exhibit both lytic and lysogenic…

Q: Outline the steps of bacterial transduction?

A: Bacteria are single-celled microorganisms. Some of them are injurious while some are beneficial to…

Q: Explain the role of viruses and its uses?

A: Viruses are small parasites and are not able to reproduce on their own. They infect the susceptible…

Q: Can viruses reproduce by themselves?

A: A virus is an infectious microorganism made up of a nucleic acid segment (DNA or RNA) encased in a…

Q: impacts might mutations have on the transmission of the virus?

A: A mutation is a change  that occurs in our DNA sequence, either due to mistakes when the DNA is…

Q: Explain how gene therapy was used to treat SCID in a patient

A: Severe Combined Immunodeficiency Disease (SCID) is a rare genetic disorder characterized by the…

Q: Discuss the origin and importance of viruses.

A: Viruses are much smaller and simpler than prokaryotic cells and are responsible for some of the most…

Q: a method a virus can use to prime DNA replication and give an example of a virus that uses this…

A: Viral Replication of double stranded DNA proceed in two phases first replication of early phase…

Q: How can viruses cause mutations?

A: Viruses are microscopic infectious agents that have a DNA or RNA surrounded by a protein coat, and…

Q: Define how DNA can be inserted into vectors ?

A:

Q: Describe the difference between lytic and lysogenic bacteriophage.

A: Bacteriophages are the virus particles that have the ability to invade and affect the bacterial…

Q: What types of viruses are used for gene therapy, and how are they manipulated to make them useful?

A: Gene therapy is the technique used to cure or prevent the disease by introducing the normal gene in…

Q: Describe how lytic bacteriophages are released from their host cells.

A: A temperate phage has an option to undergo lytic and lysogenic cycle. A lytic cycle involves the…

Q: What does it mean when you call a virus "selective"?

A: The virus is a very tiny infectious agent, which becomes active only if it gets into a host cell.…

Q: Explain Packaging recombinant DNAs into retrovirus particles?

A: Answer: Introduction: A retrovirus consists of RNA as a genetic material, it is inserted in to the…

Q: Why are viruses used in gene therapy?

A: Gene therapy is a procedure that modifies a person's genes in order to treat or cure a disease. Gene…

Q: Explain the Structure of the AIDS virus?

A: AIDS is Acquired Immune Deficiency Syndrome.

Q: Why is the detection of viral RNA not equivalent to a high risk transmission method?

A: The detection of the viral RNA, detection of infectious virus and equivalent transmission risk are…

Q: What are the potential concerns regarding the use of viral vectors in gene therapy?

A: Introduction :- Gene therapy is the process of changing the genes in your body's cells in order to…

Q: Explain the Life cycle of the AIDS virus?

A: Viruses are infectious agents with a very different organization as well as reproduction. A complete…

Q: Which viruses have potential for the highest mutation rates, DNA or RNA viruses? Explain

A: DNA viruses and RNA viruses have deoxyribose nucleic acid and ribonucleic acid, respectively. These…

Q: Define human endogenous retroviruses (HERVs)?

A: Retroviruses are single stranded, positive sense, enveloped, icosahedral and have the reverse…

Q: Why is it easier to target a single mutant versus multiple mutants in the virus?

A: Viruses are ceaselessly changing because of hereditary determination. They go through hereditary…

Q: Viral gene delivery vectors are favored for therapeutic applications because of their efficiency and…

A: Viral gene delivery vectors or the viral vectors are the most preferred method of gene transduction…