Explain the Choice of vectors?

Q: name  the six different types of vectors?

A: Vector is DNA molecule that is used as a vehicle to artificially carry the gene of interest using…

Q: Describe at least five (05) different types of commercially available vectors for cloning with size…

A: Cloning vectors are small DNA parts that are stably maintained in an organism. The foreign fragment…

Q: List the three features of a cloning vector and why these elements are needed

A: Cloning vectors act as a vehicle to transfer desired foreign genetic material into a cell. All these…

Q: discuss the factors to consider when choosing vectors for gene transfer

A: Vectors are the carrier DNA molecules that carry the gene of interest and are transferred into other…

Q: Using illustrations describe the structure of a typical cloning vector and discuss the functions of…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: When you know the sequence of the gene for the ocular protein where would you get the DNA you need…

A: The cloning is the method of recombinant DNA technology in which a piece of known DNA sequence is…

Q: Which of the following viruses have been used as vectors for gene delivery?

A: Gene delivery is a process of introducing the foreign genetic materials which are DNA and RNA into…

Q: Mention any three vector-less methods that are used to introduce recombinant DNA into a competent…

A: Micropropagation ,electroporation and gene gun methods are three vectorless methods that are used to…

Q: define wild-type ORF

A: The genes are located on the DNA which gets transcribed into mRNA through the process known as…

Q: Give method of breeding for disease resistence?

A: Plant breeding is a method of introducing favorable genes into the desired species. The favorable…

Q: Match the photo to the name of the vector

A: A vector is a living organism that transmits an infectious agent from an infected animal to another…

Q: How is gene Z used as a marker?

A: β-galactosidase, also known as lactase, beta-gal or β-gal, is a glycoside hydrolase  enzyme which…

Q: Name several types of vectors, and list the types of genes that they can contain.

A: The vector is a DNA molecule that serves as the carrier for the transfer or insertion of foreign…

Q: Explain how the frequency of recombination may be utilized to create a genetic map.

A: A gene is sequence of nucleotides in RNA or DNA often found on a chromosome. It encodes the…

Q: What are ‘Selectable marker’? What is their use in genetic engineering?

A: Genetic engineering is a technique to manipulate the genetic content of an organism resulting in…

Q: xplain any three methods of vector less gene transfer.

A: In a molecular cloning method, a vector is any vehicle, most commonly a virus or plasmid, that is…

Q: Mention the important properties which a good vector must possess?

A: The vectors that are commonly used in the field of genetic engineering possess certain features that…

Q: Explain ORF analysis.

A: Protein translocation is a process where proteins move between cellular components. It can occur…

Q: What is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

A: Gene therapy is a method that allows the correction of the defective gene. In gene therapy, it is…

Q: What is the difference between generalized and specialized transduction?

A: Transduction is a method of gene transfer in bacteria from donor to recipient using bacteriophage.…

Q: Using the question in the image below, identify the locations of each mutation in strains 1, 2, and…

A: Genes are short segments of DNA. They are responsible for our attributes, which are things like hair…

Q: What is preimplantation genetic diagnosis?

A: Prenatal diagnosis is performed for the identification of genetic diseases and their predisposition…

Q: Define how DNA can be inserted into vectors ?

A:

Q: Why is  Neurospora a good test organism?

A: Neurospora is a genus of fast-growing orange-red fungus, which lives mainly in tropical and…

Q: Explain the several key properties of DNA vectors ?

A: Microbiology is the scientific study of single celled, multicellular, and acellular microorganisms.…

Q: Describe the process of Southern blotting.

A: Introduction: Southern blotting works on the principle involving the separation of fragments of DNA…

Q: explain Generalized transduction using diagram

A: Transduction is the process by which foreign genetic material (DNA) is introduced inside the viral…

Q: What is the major difference between generalized transductionand transformation?

A: TRANSDUCTION:- Is the process  by which DNA is transferred from one bacterium to another by a virus…

Q: A. What is random mutagenesis? B. How many people a year die due to insecticide misuse and exposure?

A: Selective breeding practises having existed for crops ever since man started cultivation. So there…

Q: How is a genome-wide association study carried out?

A: Deoxyribonucleic acid (DNA) is a genetic material of most organisms. DNA contains the instructions…

Q: Contigs are often made using BAC or cosmid vectors. What are theadvantages and disadvantages of…

A: A contig refers to a set of overlapping segments of DNA (deoxyribonucleic acid) together forming a…

Q: Describe any three vectors less method of introducing the rDNA into a competent host cell?

A: rDNA is the recombinant DNA which is formed by combining desired genes from different organisms. It…

Q: What are selectable marker genes ? Explain the role of selectable marker gene ?

A: Seletable marker Genes are present in the plasmid and they are help in selection if…

Q: what is the linkage between the ct and s genes?   A. 15.7 cM   B. 32.6 cM   C. 21.3 cM   D.…

A: Genes that are sufficiently close together on a chromosome will tend to "stick together," and the…

Q: Diagram and explain how an A/G SNV can be distinguished using a left apex probe. Show the results…

A: A single-nucleotide polymorphism (SNP) occurs when a single nucleotide in the genome gets…

Q: Which vector is used to replace the defective gene in gene therapy?

A: Gene therapy focuses on utilizing the nucleic acids as a drug to treat cancer and other genetic…

Q: What is a vector? Give an example of a vector.

A: Microbiology is the study of living organism which cannot be seen through naked eyes. These…

Q: Draw a simplified schematic for Heidelberg screening to identify zygotic genes.

A: Zygotic mutants P     z/+ ♀      x      z/+♂ F1    z/z           z/+    z/+   +/+

Q: What is a transplant experiment? What is a common garden? What is the point of using these two types…

A: BASIC INFORMATION  ECOLOGY  When the relationship between the organisms living on the earth and…

Q: Describe two methods for transforming a vector into bacteria.

A: Transformation Transformation is the transfer of genetic information through the agency of free DNA…

Q: What error leads to generalized transduction?

A: Horizontal gene transfer (HGT) is the transfer of genetic information between unicellular and/or…

Q: What is a gene gun used for?

A: Step 1 A gene gun or a biolistic particle delivery system is a device that can transform almost any…

Q: What is selectable marker gene?

A: The microbial culture technique is the method of multiplying the microbial organism under controlled…

Q: Describe the uses of vectors known as YACs, BACs, and PACs.

A: Vectors are the DNA molecules that serve to carry the desired gene from the donor cells to the host…

Q: Explain the different vectors are available for cloning ?

A: Cloning vectors are the DNA molecules in which the desired DNA segments are inserted.

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.