Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.

Q: Why is it important to use standards or to develop a standard curve in spectrophotometry?

A: Spectrophotometry is the analytical technique by which the character of a substance is measured…

Q: Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron,…

A: There are different types of microscopy like bright-field, phase-contrast, dark-field, fluorescence,…

Q: When is fluorescence used in a clinical laboratory?

A: Fluorescence is a type of photoluminescence where light is absorbed by an excited molecule or atom…

Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.

A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…

Q: Describe the mechanism of SERS( Surface Enhanced Raman Spectroscopy).

A: Introduction: Surface-enhanced Raman spectroscopy (SERS) is a strong microscopic observation…

Q: What particular features of fluorescence make it useful for microscopy?

A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: What is Fluorescence Reflectance Imaging (RFI)? Explain

A: Given: Explain Fluorescence Reflectance Imaging. It is one of the imaging technique to find the most…

Q: Discuss about What is contrast harmonic Imaging?

A: Benefits of this technique are enhanced contrast resolution between normal and diseased tissues…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: What are the types of image receptor holders that can be used with the bisecting technique?

A: The bisecting angle technique: Used in dental radiology, it is an alternative to the paralleling…

Q: Discuss about Fluorescencemicroscopy

A: Fluorescence refers to the light emission by an object that contains absorbed light or any…

Q: Compare AAS, AES and UV-Vis spectrophotometer

A: The most sophisticated spectrophotometers are AAS, AES and UV-vis spectrophotometers. They each have…

Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?

A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…

Q: Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk…

A: Microscope: It is an optical instrument that uses a lens or a combination of lenses to produce…

Q: Discuss the concept of spectrophotometry, colorimetery, and turbidimetry. Maximum of 150 words

A: Spectrophotometry is a part of electromagnetic spectroscopy worried about the quantitative…

Q: principle of basic spectrophotometer

A: Spectrophotometer is an advanced instument which measures the amount of light absorbed by a sample.…

Q: What is the total magnification possible with a 10x objective?

A: The microscope is used to view the objects that are not seen through the naked eye. Microscopes use…

Q: spectrophotometer.

A: Definition:  It is defined as the branch of electromagnetic spectroscopy which is concerned with…

Q: How can one obtain 2,000× magnification with a 100× objective?

A: Magnification means how many times the image is larger than the object.

Q: What is the reason for the decrease in the ABS value over time when measuring in the…

A: A spectrophotometer is a device used in an analytical chemistry lab to measures the amount of light…

Q: What is the advantage of phase-contrast microscopyover bright-field microscopy?

A: Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light…

Q: What is the importance of sllid width in fluorescence spectroscopy

A: Fluorescent microscopy is often used to image specific features of small specimens such as microbes.

Q: Provide an illustration and brief description of the pathway of light in relation to magnification…

A: Introduction :- A microscope is a device that magnifies images of small objects, allowing the…

Q: What is an electron micrograph? Why do electron micrographshave greater resolution than light…

A: Microscope helps to observe the organisms or objects that cannot be seen by our naked eye because…

Q: Explain the difference between a transmission electronmicrograph (TEM) and a scanning electron…

A: A microscope is a laboratory instrument that is used to examine objects that are too small for the…

Q: What major advantage does phase-contrast microscopy haveover staining?

A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…

Q: spectrophotometer converts light energy into electrical energy?

A: ANS-1 Option B Explanation- Visible light is the only light our eyes can see. Our eyes detect these…

Q: Give two factors that may affect a good gram staining technique output. Provide an explanation how…

A: Many factors can affect the results of gram staining. However, two important factors that may affect…

Q: Does it enhance the meaning of work using Titan 80-300 Cubed Microscope?

A: Titan 80-300 Cubed microscope is one of the most advanced and powerful electron microscopes. It has…

Q: Draw a schematic diagram of a double beam fluorescence spectrophotometer.          Explain the…

A: Fluorescence spectrophotometer: It is a type of electromagnetic spectroscopy which analyzes…

Q: What is the working principle of raman spectroscopy.

A: Raman spectroscopy helps in determining both qualitative and quantitative information of the analyte…

Q: What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail…

A: Errors and limitations of gel electrophoresis.

Q: Introduce and explain comprehensively  the process of calculating the final magnification of…

A: A microscope is a device that magnifies an object so that the spectator can see it. A microscope is…

Q: Explain in detail simple and differential staining and also improving and adjusting contrast in…

A: Staining is a chemical method which is used to colour the organism or the cell so that it is viewed…

Q: When performing a negative stain, how does it work and why does it only stain the background?

A: * Negative stain is practice of applying heavy metal salt stain to sample for observation hence…

Q: is the reason for using blind tube in spectrophotometric and calorimetric studie

A: A method which is used to  measure how much a chemical substance absorbs light by measuring the…

Q: Explain the difference between resolution and mag­nification.

A: Introduction :- The term'resolution' is used in microscopy to describe a microscope's ability to…

Q: What are the significance of UV/Vis spectrophotometer in the experiment complexation.

A: UV/VIS spectrophotometer is the technique used to determine the optical properties  such as…

Q: What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical…

A: Microscope is the widely used laboratory instrument in the molecular biology, it is mainly used to…

Q: What is the stationary and mobile phase of spectrophotometry?

A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…

Q: Discuss the types of interferences in atomic absorption spectrophotometry.

A: Discuss the types of interferences in atomic absorption spectrophotometry. Introduction:  Spectral…

Q: Provide a list of the names of the devices and technologies used in Dynamic Light Scattering applied…

A: Dynamic Light Scattering (DLS) employs a measurement method to identify the particle size in…

Q: How does differential interference contrastmicroscopy differ from bright-field microscopy?

A: Microscopes are used to observe objects at cellular level such as the nucleus, mitochondria and…

Q: Explain situations in which pulsed-field gel electrophoresis is most useful.

A: Numerous bacteria cannot be cultivated using the same genotypic techniques, according to genetic…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.