Question
Asked Oct 17, 2019

Explain the steps of Bacterial Translation Initiation. 

1. Formation of preinitiation complex. 

2. Formation of 30S initiation complex. 

3. Ribosome assembly. 

check_circleExpert Solution
Step 1

The translation is defined as the process in which information in messenger RNA is decoded to produce a new polypeptide chain. The codons present in mRNA provides the information for protein synthesis. The translation process is divided into three parts: initiation, elongation, and termination.

Step 2

1. Formation of preinitiation complex. 

The 70SIC (70 S initiation complex) is the preinitiation complex formed  before the translation process. The complex is formed by joining of ribosomal subunits. Translation occurs on small molecules known as ribosomes.  Ribosome is composed of smaller and larger subunit that decodes the mRNA transcript and generates the polypeptide chain.

The initiation factors IF1, IF2, IF3 assist the binding of smaller subunit with mRNA transcript, they also mediate base pairing between the start codon and anticodon of tRNA. 

Step 3

2.Formation of 30S initiation complex:

The process of transcription begins with the formation of initiation complex known as 30 S initiation complex (30SIC). The complex consists of initiation factors IF1, IF2 and1F3, mRNA start codon and an anticodon.  

The start codon of mRNA trans...

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