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pls do not copy paste
Explain the steps of preparing a cell culture stock from a T25 flask.
Step by step
Solved in 2 steps
- Compare the principle behind DNA migration in an agarose gel electrophoresis from thatof protein migration in an SDS-PAGE.Describe how Blue/White screening reagent helps in screening the colonies of transformed cells.Give typed full explanation Antibiotic sensitivity.What is relationship to ellipse size and sensitivity for an E-Test?
- Fluorescence in-situ hybridisation (FISH) analysis can be performed on fixed pathological tumour sections. Briefly outline why interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISHGeneralized transduction can transfer: please explain the answer a.any DNA from the host genome b.only phage-specific DNA sequences c.genes flanked by insertion elements d.only genes near the site of integrationUsing a schematic diagram, summarize the following steps in preparing competent cells for transformation: Inoculate a single colony of E. coli into 5 ml LB broth and incubate overnight at 37°C with moderate shaking (250 rpm). Add 200 μl of the culture into 50 ml LB broth and incubate overnight at 37°C with moderate shaking (250 rpm) to an OD600 = 1.3 to 1.5. Aliquot culture into five 15-ml pre-chilled, conical tubes. Leave tube on ice 5 to 10 min. Centrifuge cells 7 min at 1,600 × g (3,000 rpm), 4°C. Pour off supernatant and resuspend each pellet in 10 ml ice-cold CaCl2 solution (50 mM CaCl2), perform resuspension very gently, and keep on ice. Centrifuge cells 5 min at 1,100 × g (2,500 rpm), 4°C. Discard supernatant and resuspend each pellet in 10 ml ice-cold CaCl2 solution. Keep resuspended on ice for 30 min. Centrifuge cells 5 min at 1,100 × g, 4°C. Discard supernatant and resuspend each pellet in 10 ml ice-cold CaCl2 solution. Dispense cells (250 μl) into pre-chilled, sterile…
- isolation and purification of bacteriophages from sewage through enrichment centrifugation and the plaque plate technique? lab reeportTrue or false White colonies in apicillin-agar plates using plasmids with lacZ gene as vector indicated both successful transformation and insertionGive advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.