Explain why litmus milk is considered a good differential medium.

Q: Illustrate the step by step method of preparing culture media.

A: Introduction Culture medium:- It is a liquid or gel designed for specific mixtures of nutrients and…

Q: Why is dilution a necessary part of pure culture preparation?

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: Explain the purpose of thiosulfate in the medium.

A: Culture media is used to enrich the number of bacterial growth, to select certain bacteria and…

Q: If the pH indicator were left out of MacConkey agar, the medium would bea) complex.b)…

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…

A: Generation time is the time which is taken by the bacteria to double in number during a specified…

Q: Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.

A: Escherichia coli is the gram negative bacteria.

Q: Why is the culture medium referred to as a highly enriched laboratory soil?

A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…

Q: Why del factor is important in media sterilization?

A: Sterilization, which was called by various means such as Del factor, Nabla factor and the…

Q: Define sterilization.

A: Microbial control indicates the prevention or inhibition of microbial growth. It involves physical…

Q: What are the approaches for sterilization during the production of sterile products? Please answer…

A: destruction or complete removal of all living organisms and their spores is called as sterilization.…

Q: The following media are used to grow bacteria for various purposes. Match the examples of media that…

A: MacConkey Agar: - Selective Growth Medium Malt Extract Agar:- Complex Medium

Q: The culture medium can be reffered to as a 'highly enriched labotary soil.justify this statement?

A: Culture medium:   It is also known as a growth medium where specific microorganisms and plants will…

Q: Agar is a solidifying agent used in media preparation. What is its origin?

A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…

Q: What is a pure culture?

A: Introduction  Culture is referred to th process of growing the cell outside the animal or plant body…

Q: Explain the purpose of the phenol red in the medium.

A: Phenol red is a water soluble dye that functions as a pH indicator in culture media. Phenol red…

Q: Define a pure culture.

A: A microbiological culture, or microbial culture, is a strategy for duplicating microbial life forms…

Q: Name the source from where agar-agar is obtained.

A: Agar-agar is a gelatinous substance that is formed by a combination of linear polysaccharide agarose…

Q: Why have several specialized media been developed? Give an example ofone such medium.

A: Culture media is of basic importance for many microbiological tests: to get pure cultures, to grow…

Q: Please refer to the figure of an agarose gel below to answer the questions. Why is it meant by the…

A: Agarose gel electrophoresis is a fundamental technique in molecular biology that is used to view and…

Q: State four reasons why barley is terminated in beer brewing process.

A: Beer brewing:         The process of production of beer by steeping a starch source in water,…

Q: The factors for the manipulation of culture medium?

A: Culture medium It is also called a growth medium. It is a solid, liquid, or semi-solid medium that…

Q: why macconkey agar and mannitol salt are regard as salective and deffrental medium?

A: A culture medium is just water and nutrients that support microbic growth. Primary culture media for…

Q: Why does turbidity in a cultured media losses its reliability at high cell concentrations?

A: turbidity is used as a measure of cell density within a culture sample.

Q: Why is agar ideal for microbial cultivation?

A: The microbial cultivation is the process in which microorganisms are cultivated in the laboratory,…

Q: Explain why it is important to use only a small amount of bacteria when preparing a smear

A:

Q: give one reason why is it necessary to keep stirring the agar medium mixture in a beaker when…

A: Agar is a heteropolysaccharide, which is composed of different carbohydrate units. Agar is obtained…

Q: The most probably number (MPN) method for measuring Nitrifiers in soil Microbiology ?

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: What is a pure culture and how can one be obtained

A: Cells are the structural and functional units of life.

Q: What is the advantage of the plating method over an electronic cell counting method in counting…

A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…

Q: What process would explain why the dyes spread through the agar?

A: Agar -- Agar is polymer and mixture of polysaccharides obtained from the cell wall of red algae .…

Q: medium in which all the components are known is: A. Minimal medium B. Synthetic or defined medium…

A: Microorganisms are small, unicellular, and microscopic organisms. It is important to grow…

Q: You were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent.…

A: The dilution factor can be defined as the ratio of Volume of the initial solution to the volume of…

Q: Distinguish batch culture and continuous culture

A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…

Q: Briefly describe the phenol coefficient test.

A: Sterilization is a process that involves the removal of harmful microbes either by inhibit the…

Q: What compounds or chemicals might be added to a medium to make it selective?

A: Culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth of…

Q: Agar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal…

A: A growth medium or culture medium is a gel or liquid designed to support microorganism growth. The…

Q: If my cultures are suffering severely due to alkaloid production and accumulation in the medium. In…

A: Alkaloids are basically the natural substances that can be used largely for human medication.…

Q: Explain the rational of using saponification-solvent extraction to extract pyrene from milk

A: Asked : Rational of using saponification-solvent extraction to extract pyrene from milk

Q: Which among the ingredients of this product is the main preservative? Explain.

A: For centuries, people have been preserving food. It all began with the ancient practice of…

Q: Sugar Utilization medium (Containing trehalose, sucrose, trehalose, Mannose,etcUrease

A: The medium asked in the question is a fermentation medium used in carbohydrate fermentation test…

Q: If testing had come back as oxidase negative, urease positive and growth on MacConkey agar, what…

A: MacConkey agar is a selective and differential culture medium used for the isolation and…

Q: In the tripple sugar fermentation test, why is the triple sugar iron agar called a multi-test…

A: The process of chemical breakdown of complex substances into simple ones in the absence of oxygen…

Q: Give the components, properties and special considerations in preparation (if there's any) on the…

A: MacConkey Agar . It is selective for gram- negative rods bacteria. EMB . It is selective for fecal…

Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: Write the limitations of sterilization by Dry heat?

A: The dry heat sterilization technique includes the removal or killing the microorganism and require…

Q: What is the major difference between Thayer-Martin and chocolate agar? When would you use…

A:

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.