For a test of enzyme-catalyzed kinetics, the following data were recorded 0 product (°C) (mmoVmL) (mmol/mL-min) 30 0.1 30 0.033 30 0.02 30 0.01 30 0.005 49.6 0.1 49.6 0.033 49.6 0.01 49.6 0.0067 49.6 0.005 2.63 1.92 1.47 0.96 0.56 5.13 3.70 1.89 1.43 1.11 a. Determine the M-M constants (VMAx and KM) and the specific rate of product formation (kcaT) for the reaction at 30 oC and at 49.6 °C. Use the Lineweaver-Burke plot to determine the constants, b. Demonstrate the effect of the temperature change from 30 °C to 49.6 °C by plotting the measured reaction rate vs. the substrate concentration (a plot of the M-M curves). Indicate that the enzyme activity is in the temperature activation region or in the temperature deactivation region

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