Homework Help is Here – Start Your Trial Now!
Engineering
Chemical Engineering
For a test of enzyme-catalyzed kinetics, the following data were recorded 0 product (°C) (mmoVmL) (mmol/mL-min) 30 0.1 30 0.033 30 0.02 30 0.01 30 0.005 49.6 0.1 49.6 0.033 49.6 0.01 49.6 0.0067 49.6 0.005 2.63 1.92 1.47 0.96 0.56 5.13 3.70 1.89 1.43 1.11 a. Determine the M-M constants (VMAx and KM) and the specific rate of product formation (kcaT) for the reaction at 30 oC and at 49.6 °C. Use the Lineweaver-Burke plot to determine the constants, b. Demonstrate the effect of the temperature change from 30 °C to 49.6 °C by plotting the measured reaction rate vs. the substrate concentration (a plot of the M-M curves). Indicate that the enzyme activity is in the temperature activation region or in the temperature deactivation region

For a test of enzyme-catalyzed kinetics, the following data were recorded 0 product (°C) (mmoVmL) (mmol/mL-min) 30 0.1 30 0.033 30 0.02 30 0.01 30 0.005 49.6 0.1 49.6 0.033 49.6 0.01 49.6 0.0067 49.6 0.005 2.63 1.92 1.47 0.96 0.56 5.13 3.70 1.89 1.43 1.11 a. Determine the M-M constants (VMAx and KM) and the specific rate of product formation (kcaT) for the reaction at 30 oC and at 49.6 °C. Use the Lineweaver-Burke plot to determine the constants, b. Demonstrate the effect of the temperature change from 30 °C to 49.6 °C by plotting the measured reaction rate vs. the substrate concentration (a plot of the M-M curves). Indicate that the enzyme activity is in the temperature activation region or in the temperature deactivation region

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
See similar textbooks
icon
Related questions
Question

All details in question, please show all steps

For a test of enzyme-catalyzed kinetics, the following data were recorded 0 product (°C) (mmoVmL) (mmol/mL-min) 30 0.1 30 0.033 30 0.02 30 0.01 30 0.005 49.6 0.1 49.6 0.033 49.6 0.01 49.6 0.0067 49.6 0.005 2.63 1.92 1.47 0.96 0.56 5.13 3.70 1.89 1.43 1.11 a. Determine the M-M constants (VMAx and KM) and the specific rate of product formation (kcaT) for the reaction at 30 oC and at 49.6 °C. Use the Lineweaver-Burke plot to determine the constants, b. Demonstrate the effect of the temperature change from 30 °C to 49.6 °C by plotting the measured reaction rate vs. the substrate concentration (a plot of the M-M curves). Indicate that the enzyme activity is in the temperature activation region or in the temperature deactivation region
Transcribed Image Text:For a test of enzyme-catalyzed kinetics, the following data were recorded 0 product (°C) (mmoVmL) (mmol/mL-min) 30 0.1 30 0.033 30 0.02 30 0.01 30 0.005 49.6 0.1 49.6 0.033 49.6 0.01 49.6 0.0067 49.6 0.005 2.63 1.92 1.47 0.96 0.56 5.13 3.70 1.89 1.43 1.11 a. Determine the M-M constants (VMAx and KM) and the specific rate of product formation (kcaT) for the reaction at 30 oC and at 49.6 °C. Use the Lineweaver-Burke plot to determine the constants, b. Demonstrate the effect of the temperature change from 30 °C to 49.6 °C by plotting the measured reaction rate vs. the substrate concentration (a plot of the M-M curves). Indicate that the enzyme activity is in the temperature activation region or in the temperature deactivation region
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 8 steps with 8 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Flow past immersed solids
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemical-engineering and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Introduction to Chemical Engineering Thermodynami…
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Process Dynamics and Control, 4e
Process Dynamics and Control, 4e
Chemical Engineering
ISBN:
9781119285915
Author:
Seborg
Publisher:
WILEY
Industrial Plastics: Theory and Applications
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
Unit Operations of Chemical Engineering
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The
SEE MORE TEXTBOOKS