For a test of enzyme-catalyzed kinetics, the following data were recorded 0 Tproduct (mmol/mL) (mmol/mL) (mmol/mL-min) 0.92 0.92 0.92 0.92 0.92 0.92 30 30 30 30 30 30 0.100 0.020 0.010 0.100 0.033 0.020 1.64 0.90 0.58 1.33 0.80 0.57 0.6 0.6 0.6 a. Determine the constants Vmax and Km at 30 °C and an enzyme concentration of 0.92 mmol/mL for the uninhibited reaction and for the inhibited reaction. b. Indicate what type of inhibition is present in the reaction. Use the appropriate kinetic equation for the type of inhibition present to solve for the constant of inhibition in the equation using your solved values of Vmax, Km, and one point of data from the table for the remaining terms in the equation.
For a test of enzyme-catalyzed kinetics, the following data were recorded 0 Tproduct (mmol/mL) (mmol/mL) (mmol/mL-min) 0.92 0.92 0.92 0.92 0.92 0.92 30 30 30 30 30 30 0.100 0.020 0.010 0.100 0.033 0.020 1.64 0.90 0.58 1.33 0.80 0.57 0.6 0.6 0.6 a. Determine the constants Vmax and Km at 30 °C and an enzyme concentration of 0.92 mmol/mL for the uninhibited reaction and for the inhibited reaction. b. Indicate what type of inhibition is present in the reaction. Use the appropriate kinetic equation for the type of inhibition present to solve for the constant of inhibition in the equation using your solved values of Vmax, Km, and one point of data from the table for the remaining terms in the equation.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Transcribed Image Text:For a test of enzyme-catalyzed kinetics, the following data were recorded
0
Tproduct
(mmol/mL) (mmol/mL) (mmol/mL-min)
0.92
0.92
0.92
0.92
0.92
0.92
30
30
30
30
30
30
0.100
0.020
0.010
0.100
0.033
0.020
1.64
0.90
0.58
1.33
0.80
0.57
0.6
0.6
0.6
a. Determine the constants Vmax and Km at 30 °C and an enzyme concentration of 0.92
mmol/mL for the uninhibited reaction and for the inhibited reaction.
b. Indicate what type of inhibition is present in the reaction. Use the appropriate kinetic equation
for the type of inhibition present to solve for the constant of inhibition in the equation using your
solved values of Vmax, Km, and one point of data from the table for the remaining terms in the
equation.
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