For the Complex III in the electron transport chain: Complex III step 1: UQH2 is oxidized in a 2 electron process. Cytochrome c is reduced and UQ is reduced to UQH in two 1 electron processes. Complex III step 2: UQH2 is oxidized in a 2 electron process. Cytochrome c is reduced and UQH is reduced to UQH2 in two 1 electron processes. The necessary standard reduction potentials are: UQ + 2H+ + 2e- UQH2 E° = 0.06 V cyt c (Fe3+) + e- cyt c (Fe2+) E° = 0.254 V UQ + H+ + e- UQH. E° = 0.03 V UQH. + H+ + e- UQH2 E° = 0.19 V Calculate the total redox potential of the complex. Now calculate how many moles of protons can be translocated across the inner mitochondrial membrane if translocation of 1 mole requires 23 kJ. Calculate the free energy available for proton translocation assuming a 2electron process for each complex.
For the Complex III in the electron transport chain:
Complex III step 1: UQH2 is oxidized in a 2 electron process. Cytochrome c is reduced and UQ is reduced to UQH in two 1 electron processes.
Complex III step 2: UQH2 is oxidized in a 2 electron process. Cytochrome c is reduced and UQH is reduced to UQH2 in two 1 electron processes.
The necessary standard reduction potentials are:
UQ + 2H+ + 2e- UQH2 E° = 0.06 V cyt c (Fe3+) + e- cyt c (Fe2+) E° = 0.254 V UQ + H+ + e- UQH. E° = 0.03 V
UQH. + H+ + e- UQH2 E° = 0.19 V
Calculate the total redox potential of the complex.
Now calculate how many moles of protons can be translocated across the inner mitochondrial membrane if translocation of 1 mole requires 23 kJ.
Calculate the free energy available for proton translocation assuming a 2electron process for each complex.
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