From a kinetics experiment, Kcat was determined to be 55sec^-1. For the kinetic assay, 0.05mL of a 0.05mg/mL solution of the enzyme was used, and the enzyme has a molecular weight of 30,000g/mole. Assume a reaction volume of 3mL. Calculate Vmax (um*min^-1) for the enzyme and catalytic efficiency (in  M^-1sec^-1) for the enzyme. The Km for the enzyme was determined to be 8.3*10^-2M.

Biochemistry
9th Edition
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Chapter6: The Behavior Of Proteins: Enzymes
Section: Chapter Questions
Problem 31RE: MATHEMATICAL You do an enzyme kinetic experiment and calculate a Vmax of 100 mol of product per...
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Deepika_Biology

 From a kinetics experiment, Kcat was determined to be 55sec^-1. For the kinetic assay, 0.05mL of a 0.05mg/mL solution of the enzyme was used, and the enzyme has a molecular weight of 30,000g/mole. Assume a reaction volume of 3mL. Calculate Vmax (um*min^-1) for the enzyme and catalytic efficiency (in  M^-1sec^-1) for the enzyme. The Km for the enzyme was determined to be 8.3*10^-2M. 

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