Question
Asked Mar 10, 2019
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Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?

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Expert Answer

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Step 1

The following formula is used to find the volume required to prepare a 5 mL sample with a final concentration of 1 mg/mL, where C1 is the concentration of BSA stock, V1 is the required volume of stock to prepare the sample, C2 is concentration of the sample, and V2 is the final volume of sample.

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Step 2

Given:

C1 = 4mg/mL

V1  = ?

C2 = 1 mg/mL

V2 = 5 mL

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Step 3

Each 5ml of sample used in the reaction will contain 1.25ml of BSA stock and 3.75 ml of water in orde...

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