Given: Your professor gives you a vial of enzyme and a vial of substrate. The product of this reaction is fluorescent and you can measure the concentration of the product as a function of time.(answer a, b, and c)a) Your professor tells you to quantify how much product is being produced per minute at thebeginning of the experiment. What exactly are you measuring?b) On the same plot, show the kinetic curve for an increased quantity of enzyme. Assume a 2x concentration. Indicate Km and Vmax.c) You don’t have a computer hand. Sad. How can you plot the data such that you can get important Michaelis-Menten values?

Question
Asked Sep 24, 2019

Given: Your professor gives you a vial of enzyme and a vial of substrate. The product of this reaction is fluorescent and you can measure the concentration of the product as a function of time.

(answer a, b, and c)
a) Your professor tells you to quantify how much product is being produced per minute at the
beginning of the experiment. What exactly are you measuring?
b) On the same plot, show the kinetic curve for an increased quantity of enzyme. Assume a 2x concentration. Indicate Km and Vmax.
c) You don’t have a computer hand. Sad. How can you plot the data such that you can get important Michaelis-Menten values?

check_circleExpert Solution
Step 1

Michaelis-Menten kinetics describes the rate of enzyme-catalyzed reactions. It relates the rate of reaction (ʋ) with concentration of substrate (S) and product (P), by substituting these values in the Michaelis-Menten equation. The Michaelis-Menten equation is given below:

max
KMS]
Where,
K/ Michaelis constant
=Maximum rate achieved by the system
max
help_outline

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max KMS] Where, K/ Michaelis constant =Maximum rate achieved by the system max

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Step 2

a) Calculating the amount of product produced per minute in a given enzymatic reaction is termed as the rate of the reaction. This means, here the professor is asking the student to calculate the rate of the reaction. The formula for rate of reaction is given below:

d[P]
dt
where,
[P] Concentration of product
t total time taken to form product
help_outline

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d[P] dt where, [P] Concentration of product t total time taken to form product

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Step 3

b) The rate of the reaction and maximum rate of the reaction (Vmax) depends on the enzymes concentration in the reaction. This is because of the increase in availability of the active site of the enzyme, which can participate in the reaction by bind...

Vamx at 2X enzyme
concentration
Vmax
Vmax at X enzyme
Vmax
concentration
dP/dt
Km
help_outline

Image Transcriptionclose

Vamx at 2X enzyme concentration Vmax Vmax at X enzyme Vmax concentration dP/dt Km

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