Question
Asked Sep 26, 2019

How is phenyl isothiocyanate useful in determining protein structure?

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Expert Answer

In determining protein structure, The first step is to determine the amino acid composition of the peptide.

The peptide is heated in 6 N HCl at 110°C for 24 hours. This helps in obtaining the amino acids that the peptide is made of.

The next step is to identify the N-terminal amino acid.

Reaction of Phenyl isothiocyanate:

Phenyl isothiocyanate reacts with the uncharged termina...

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