How will the different agents precipitate egg albumin? Include chemical equations involved Heat Heavy metal salts Strong mineral acids Full-saturation test half-saturation test Alkaloidal reagents alcohol
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PRECIPITATION OF PROTEINS
How will the different agents precipitate egg albumin? Include chemical equations involved
- Heat
- Heavy metal salts
- Strong mineral acids
- Full-saturation test
- half-saturation test
- Alkaloidal reagents
- alcohol
Step by step
Solved in 2 steps with 6 images
- Accounts for less than 2 % of total albumen protein. This is the protein that makes the egg white thick when cooked. a. Ovatranferrin b. Ovomucin c. Ovalbumin The most plentiful in the egg. It is the only protein with a reactive sulfur groups, making it decisive to the flavor, texture, and color of cooked eggs. a. Ovatranferrin b. Ovomucin c. Ovalbumin This is the first protein to coagulate when an egg is cooked. Holds tightly onto iron atoms to prevent bacteria from using them. a. Ovatranferrin b. Ovomucin c. OvalbuminTest for the Components of Milk A. Test for Casein PROCEDURE: In a test tube that perform Millon’s test, place pinch of dried precipitate (casein). Observe and record the change in color. QUESTION: WHAT IS THE CONFIRMATORY TEST THAT WILL BE USED?By alkaloidal reagent Place 3mL of egg albumin solution in each of the two test tubes, to one tube add 1 – 2 ml of tannic acid solution and to other one picric acid. Describe the appearance of the protein solutions in each of the tubes after addition of the alkaloidal reagents.
- Test for the Components of Milk A. Test for Casein PROCEDURE: In two separate test tubes, place pinch of dried precipitate (casein). To the first test tube perform Biuret’s test and the second test tube perform Millon’s test. Observe and record the change in color. QUESTION: WHAT IS THE CONFIRMATORY TEST USED FOR EACH TUBE? DISCUSS AND EXPLAIN?Test for the Components of Milk A. Test for Casein PROCEDURE: In a test tube, place pinch of dried precipitate (casein). The test tube perform Millon’s test. Observe. QUESTION: WHAT IS THE CONFIRMATORY TEST USED FOR EACH TUBE? DISCUSS AND EXPLAIN?Test for the Components of Milk A. Test for Casein PROCEDURE: In two separate test tubes, place pinch ofdried precipitate (casein). To the first test tubeperform Biuret’s test and to the second testtube, perform Millon’s test. Observe andrecord the change in color. QUESTION: WHAT IS THE CONFIRMATORY TEST FOR EACH TUBE AND COLOR OF THEIR POSITIVE RESULT? DISCUSS AND EXPLAIN THE ANSWER
- Wh are doing this procedures can you explain? (ex. heating or adding chemicals etc.) 1) Purification of Vitelline from Egg Yolk-Experimental ProcedureMeasure the volume of 3 egg yolks and mix by adding an equal volume of NaCl solution.Extract the mixture with 3-fold volumes of ether and separate the aqueous phase.Do the same procedure 3 times.Mix the sample with water and rinse.It is expected for the protein to collapse.Some more water is added in order to check whether the collapse occurs completely.The sample is centrifuged and the precipitate is dried. 2) Purification of Plasma Albumin and Fibrinogen-Experimental ProcedureAdd ammonium sulfate to 10 ml plasma up to 25% saturation.Separate the collapsed fibrinogen by centrifuge.Increase the saturation level to 33% by adding (NH4) 2SO4.Separate the globulin by centrifugation.Separate prodoglobulins by increasing the saturation level to 46%.Increase the saturation level up to 64% to precipitate albumin.Separate the albumin by centrifuge…Give the advantages and disadvantages of the following dosage forms in sentence forms: ○ Tablets○ Capsules○ Syrups○ Suppositories○ EmulsionsWhy is it not advisable to use adhesive mixture if protein histological investigation are contemplated
- Test for the Components of Milk A. Test for Casein PROCEDURE: In two separate test tubes, place pinch ofdried precipitate (casein). To the first test tubeperform Biuret’s test and to the second testtube, perform Millon’s test. Observe andrecord the change in color. QUESTION: WHAT IS THE CONFIRMATORY TEST FOR EACH TUBE AND COLOR OF THEIR POSITIVE RESULT?Multiple Choicd 1. Which color is obtained when protein is treated with Ninhydrin solution? A. Purple B. White C. Yellow D. Blue 2. What is the name given to the reaction between protein and concentrated HNO3? A. Xanthoproteic Test B. Millon's Test C. Ninhydrin Test D. Biuret TestAdvantages and disadvantages of the following dosage forms: ○ Tablets○ Capsules○ Syrups○ Suppositories○ Emulsions