How would loss of the retromer coat most likely affect cargo shuttling between the Golgi apparatus and early endosome? M6P receptor would not recycle back to the Golgi Cargo shuttling would be unaffected Vesicles would not target to the endosome O M6P receptor would not bind cargo Cargo would not be released from the M6P receptor
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- Which of the following statements about the M cyclin:CDK are FALSE: It promotes nuclear envelope assembly at the end of M phase It phosphorylates the APC/C to activate it It phosphorylates microtubule binding proteins to promote spindle formation Its activity is regulated by phosphorylationProteins imported into the nucleus_____. Select one: a. are already folded and do not have a signal sequence b. are unfolded and have a signal sequence c. are unfolded and do not have a signal sequence d. have their signal sequence removed by a peptidase e. proteins are not imported into the nucleusFtsZ proteins direct the formation of a ___________ that will eventually form the new cell walls of the daughter cells. contractile ring cell plate cytoskeletom septum
- 1. Which organelles is the main calcium store for signaling? Lyosome; ER; Golgi; secretary vesicles 2. A temperature sensitive mutant yeast strains/stops dividing when shifted from. 25 degrees Celsius to 37 degrees Celsius. These cells are analyzed at different temperatures by a machine that measures the amount of DNA they contain. What would explain the behavior of the mutant? Anaphase promoting complex is inactivatend; s cdk is inactivated; m cdk is inactivate; m cycling is overexposed 3.which of the following statements about proto oncogenes is true? Proto oncogenes inhibit cell growth and division; cancer cells often contain loss of function mutations in proto oncogenes; proto oncogenes are needed for cells in normal tissues; none 4. Which of the following statements about tumor suppressor genes is true? Gene amplification of a tumor suppressor gene is more dangerous than gene amplification of a proto oncogenes; tumor suppressor genes are more highly expressed in cancer cells…what Govern Attachment ofChro?mosomes to the SpindleCytokinesis , or division of the cytoplasm to create two new daughter cells , occurs in conjunction with telophase . Examine the telophase micrographs of the onion root tip and whitefish blastodisc cells . How does cytokinesis look different for these two cells ? Explain why cytokinesis looks different between those cells
- You genetically engineered a cell to no longer contain the extracellular domain of an integrin. What is the most likely effect of losing this domain on the cell's ability to bind? It would affect the cell's ability to attach to the ECM. It would affect both the cell's ability to attach to other cells and the cell's ability to attach to the cytoskeleton. It would affect the cell's ability to attach to the cytoskeleton through adaptor proteins. It would have no effect on the cell's ability to attach to anything.Microtubules in a cell exhibit dyanimc instability at which end? positive end negative end both positive and negative ends neither the positive nor the negative ends 2. The effect of MAP2 on microtubules is similar to the effect of CapZ on microfilaments how? both stabilize the positive end of the filament both bundle the filaments in parallel bundles both increase the instability of the negative end of the filament both nucleate the formation of the filaments 3. Select all of the statements below that are true of tubulin and microtubules negative ends of microtubules end with alpha-tubulin sticking out monomers in which GTP is bound to beta-tubulin encourage the growth of the filament hydrolysis of GTP by the beta subunit stabilizes the filament all of the tubulin subunits in a microtubule are bound to GDPPart of G1 is for the cell to enlarge, this growth can cause internal signaling from desmosomes which may stop cell division. What is the connection between desmosomes and progression of the cell cycle? Select one: a. Desmosomes are linked to the MPF. When the cell grows it will turn on the MPF through the desmosomes which will turn off the APC, stopping cell division. b. Desmosomes have nothing to do with regulating the cell cycle. c. Desmosomes are linked to microfilaments that make up the cellular cortex. When pulled, they stop kinesins from walking on microtubules, halting cell division. d. Desmosomes are connected to intermediate filaments (the skeleton of the cell). As the cell grows, if the cell receives signals (mechanical through touching) from the desmosomes that there are cells on all sides, this will halt cell division at the G1 checkpoint as part of density-dependent inhibition.
- Streptomyces coelicolor is studied as a model system for cellular differentiation. Some of the genes involved in sporulation contain an AT-rich leucine codon not used in vegetative genes. Suggest how Streptomyces might use this rare codon to regulate sporulationMitotic spindle function relies heavily on microtubule motors. For each of the following motor proteins, predict the effect on spindle formation, function, or both of adding a drug that specifically inhibits only that motor: kinesin-5, kinesin-13, and kinesin-4.Need help If the mitotic spindle does not correctly attach to chromosome kinetochores, the spindle assembly checkpoint will be activated. Select all correct outcomes for chromosomes not correctly attached to mitotic spindle A) centrosome duplication will not occur B) M cyclin will be degraded C) APC will be inhibited D) chromosomes will not segregate E) Mad2 will bind to unattached kinetochores F) mitosis will halt at metaphase/anaphase transition