Q: Explain the bacterial transformation experiments of Avery and his colleagues and the results they…
A: Avery, MacLeod & McCarty, 1944 repeated Griffith’s experiment of transformation by using…
Q: How would I know if my PCR product is pure?
A: Polymerase Chain Reaction (PCR) is one of the most significant tools of molecular biology. It was…
Q: Which is the correct order of the PCR reaction?
A: Introduction PCR or the polymerase Chain Reaction is a molecular technique used to amplify a…
Q: Why is Taq polymerase especially useful for PCR?
A: PCR or Polymerase Chain Reaction is a lab technique used to make copies of a particular DNA…
Q: How does magnetic bead DNA extraction work?
A: DNA extraction is the method of isolating DNA by disrupting the cell wall and nuclear membrane with…
Q: Why carry out genetic screening at all?
A: Genes are the basic structural and functional unit of heredity. They carry coded genetic information…
Q: The cath sample was both filtered to remove all bacteria, and then treated with DNase. Based on…
A: A cath or catheter is a thin hollow tube, which is a medical equipment that is used for various…
Q: What do restriction enzymes do
A: What do restriction enzymes do ?
Q: What is the job of the restriction enzyme?
A: In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli…
Q: Why is PCR beneficial?
A: PCR is a very useful tool and has many applications in various fields such as medical, forensic,…
Q: Can we do PCR on a plasmid?
A: In molecular biology, the plasmid is most abundantly used. The plasmid is a circular autonomously…
Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?
A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…
Q: What do you mean by restriction fragments?
A: Recombinant DNA technology is the process of combining two or more DNA fragments from a different…
Q: What do you mean by Southern Blotting technique?
A: Southern blotting is a laboratory technique that is widely used to detect a specific DNA sequence in…
Q: What different techniques can be used for transcriptomeanalysis ?
A: The transcriptome is the set of all RNA transcripts including coding and non-coding in an individual…
Q: Why do bacteria make restriction endonucleases?
A: Deoxyribonucleic acid (DNA) is a double-stranded molecule, which consists of two strands of…
Q: HOW Restriction Enzymes Cutthe Genome at Specific Sites?
A: Restriction enzymes are involved in accomplishing various biotechnological processes. Mostly found…
Q: Why do eukaryotic cells do not contain restriction enzymes?
A: Restriction enzymes are used extensively in molecular biology to cut DNA. One of the unique features…
Q: What normal role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from…
A: A restriction enzyme is a protein that recognizes a particular DNA sequence and cuts the DNA at a…
Q: What is linear amplification PCR?
A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…
Q: what is the function of restriction enzymes in digesting products in PCR
A: Restriction enzymes are also known as molecular scissors because they involves in digestion of…
Q: Restriction Enzymes with LongerRecognition Sites Produce Larger _______ Fragments
A: The restriction enzyme is the molecular scissors that cut at a specific site. These are naturally…
Q: What uses of Type 1 Restriction Enzymes have if they seem to be so inaccurate?
A: Restriction enzymes are used to cut the DNA into fragments that are suitable for manipulation and…
Q: What does it mean if a restriction enzyme produces ‘sticky’ or ‘blunt’ ends? Does the restriction…
A: The sticky or blunt ends refers to the edges of DNA molecules. The sticky ends tend to overlap to…
Q: Why do restriction endonucleases not hydrolyze DNA from the organism that produces it?
A: Endonucleases are the enzymes, which help to cleave the DNA from inside that is in between the…
Q: How to determine the location of restriction sites for different enzyme ?
A: Enzymes are proteins that are present in the body and help to speed up metabolism or other chemical…
Q: Why is it important to wear gloves when setting up the PCR tubes?
A: PCR represents polymerase chain reaction which is an in vitro laboratory technique used for the…
Q: How is DNA amplified in a polymerase chain reaction(PCR) procedure?
A: Step 1 Polymerase chain reaction (PCR) sometimes also called molecular photocopying is a technique…
Q: Describe how certain restriction enzymes generateDNA fragments with sticky ends, while others…
A: Restriction enzymes are used extensively in molecular biology to cut DNA. One of the unique features…
Q: What uses do Type 1 restriction enzymes have if they seem to be so inaccurate
A: Restriction enzymes refer to the restriction endonuclease enzymes that are used to cut DNA…
Q: How do tou figure out which type of restriction enzymes to use in an experiment and what would…
A: A restriction enzyme or restriction endonuclease is an enzyme that cleaves/cuts DNA into fragments…
Q: How will you obtain purified DNA from a cell?
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: Who did discover restriction enzymes and how did they discover it? And what experiments did they use…
A: It is the one of the tools of recombinant DNA technology which is known as molecular scissors. Today…
Q: Which are the Ten Commonly Used Restriction Enzymes?
A: A restriction enzyme could be a macromolecule that acknowledges a particular, short nucleotide…
Q: What are the optimal conditions for EcoRV and Aval restriction enzymes?
A: Restriction enzymes are also known as molecular scissors since they are used in cutting specific…
Q: Who isolated Restriction enzymes for the first time?
A: Restriction enzymes, also known as restriction endonucleases, recognize specific sites and cut DNA…
Q: What is a restriction digest? What does it mean if you were given a precut DNA?
A: Restriction enzyme digestion takes advantage of naturally occurring enzymes that cleave DNA at…
Q: Whats the role of a restriction enzyme?
A: Restriction enzyme is an enzyme used in recombinant DNA technology for the purpose of cloning DNA.…
Q: How do you figure out which type of restriction enzymes to use in an experiment and what would…
A: Restriction enzymes are those that cleaves the DNA into fragments at or near specific sites within…
Q: In the third step of the PCR, the temperature is raised to 73 degrees C. What happens during this…
A: PCR: PCR (polymerase chain reaction) is a technique used to make multiple copies of a particular…
Q: Vhat is needed from the cells for PCR?
A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…
Q: are restriction endonuclease enzymes named? Write examples.
A: Restriction enzymes break DNA at or near certain recognition sequences known as restriction sites,…
Q: What was the purpose for doing the colony PCR?
A: Colony PCR is a method done to screen the colonies of yeast or bacteria that have grown with the…
Q: Based on the knowledge you gained from the cloning module, which of the bands in the figure is…
A: Cloning modules allows you to have more control over which parts of the original module are…
Q: what restriction enzymes could be used to cut cDNA produced ?
A: DNA-cutting enzymes are restriction enzymes. Every enzyme detects a single or some few target…
Q: How do you genetically modify an organism? Name and describe the different methods
A: GENETICALLY MODIFIED ORGANISMS(GMOs)- They primarily denote crop plants developed for human or…
Q: Why don’t bacteria cut up their own DNA when they produce restriction enzymes?
A: Restriction enzymes are the proteins which can cut double stranded DNA segments only at some…
Q: What are the steps of a PCR?
A: PCR stands for Polymerase Chain Reaction. It is a technique of molecular biology which is used to…
How would you ascertain if your restriction experiment was successful?
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- What is a restriction digest? What does it mean if you were given a precut DNA?How do you figure out which type of restriction enzymes to use in an experiment and what would happen if they were damaged and didn't work?Explain how you would use the blue-white colony screening technique to identify bacteria containing the recombinant plasmid