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How would you dilute a stock solution of bacteria with an OD600 of 2.5 down to a solution that has an OD600 of 0.25?
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- Why is E. coli O157:H7 an organism of concern in contaminated foods? The strain is represented as O157:H7. Which particular “parts” of the bacterial cell do the “O” and “H” refer to?given a phage lysate with 10^11 pfu/ml and a bacterial culture with 10^8 cfu/ml, how would you obtain MOI =0.1 ?Why do you think most bacteria grown in labs are mesophiles with a pH optimum for growth near 7.0, do you believe that it is representative of bacteria on earth? What problems would this cause.
- What is G+C content of a bacteria? How it can be determined? Why it is not safe to assume that microorganisms with same G+C content belong to same species? How G+C content data is taxonomically valuable?You dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?Give an example of an industrial/clinical setting where quantifying viable bacteria would be useful. How would you prepare a series of dilutions to get a final dilution of 10–10? Outline each step.
- What are the disadvantages of using the mouth in filling a pipette? Why do we use exponential notation in counting bacteria? Why is serial dilution necessary?One hundred microliters (0.100 ml) of a 10-5 dilution of a bacterial suspension plated on an agar plate gave rise to 229 distinct colonies. What was the concentration of bacteria in the original, undiluted suspension?Pseudomonas bacteria have porin proteins, are resistant to the chemical triclosan, and survive and can even multiply in quaternary ammonium compounds. True or False?