How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material?  Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.

Q: What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is…

A: Methyl red test This test usually aims to differentiate the facultative anaerobes enteric bacteria.…

Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…

A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…

Q: Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer

A: Disclaimer: “Since you have asked multiple questions, we will solve the first question for you. If…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…

A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.

Q: Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when…

A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…

Q: How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of…

A: Dilution is the process of decreasing the concentration of a solute in a solution. To dilute a…

Q: What are the advantages of the API 20E test compared to regular biochemical tube media

A: API 20E test helps in the determination of bacteria from lab-scale activated sludge treatment…

Q: How to do cellulolytic characterization using a 96-well plate? What is the protocol or procedure to…

A: Cellulolytic action can be measured by an assortment of techniques that have been summed up in…

Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?

A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…

Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?

A: Utilising the membrane filter method, test is gone through the layer utilising a filter funnel and…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Why do the results for the confirmed test represent  faecal coliform in the analysis of water using…

A: MPN is Most probable number. It is commonly applied for testing the quality of water.

Q: Which is the better reagent for the clinical test between Fehling's solution and Benedict's…

A: The reducing sugars get oxidized by reducing some other component. This reducing property of these…

Q: Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that…

A: Autoclaving works on the principle of moist heat. Autoclaving is the only process that achieves…

Q: ) Gel filtration chromatography on Sephadex G50 b.SDS-PAGE

A: Gel filtration: with this method we could separate  protein with respect to molecular weight in the…

Q: When is sterilization by filtration preferred over sterilization by heat? Why? What makes heat an…

A: Answer :- 1)Filteration is the sterilization process wherein there is the physical removal of…

Q: What is the fluid thioglycollate medium? Please explain the testing and its results and the…

A: Microbiology laboratory uses various kinds of culture media to identify the biochemical…

Q: Why should Erlenmeyer flasks hold not more than 60% of their capacity?  When can gelatin be used in…

A: 1*Erlenmeyer flasks are used to contain liquids and for mixing and heating and cooling, incubation…

Q: A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture…

A: A growth medium is intended to promote the growth of a variety of microorganisms or cells by the…

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: Why is it important that the agarose is exposed to ethidium bromide, which is either added to the…

A: Ethidium bromide is utilized during separation of fragments of DNA by agarose gel electrophoresis.…

Q: You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the…

A:

Q: Calculate the IV rate in drops per minute for a 1 L bag of Lactated Ringers solution running at…

A: Drop factor refers to the number of drops that are required to make one ml of liquid. Here the drop…

Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…

A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…

Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…

A: Solid media is the type of media that is made up of liquid substances like distilled water and other…

Q: What is the basic principle of GPC (gel permeation chromatography)?

A: Gel permeation chromatography is a type of size-exclusion chromatography that uses organic solvents…

Q: What is the purpose of the sodium thiosulphate in the testing of treated water?

A: Testing of wastewater and treated water both demand a standardized process to be followed to obtain…

Q: What is one advantage of a pour plate over a streak plate?

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…

A: Column chromatography is a purification method that uses the polarity of compounds for the…

Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

A: Introduction: A CFU stands for colony-forming units. It is a unit that we use for estimating the…

Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…

A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…

Q: For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise…

A: The preparing a slide that has to be observed under a microscope there are multiple steps that are…

Q: Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that…

A: The countable range of colonies for an ideal plate count method is considered 30-300 colonies per…

Q: 100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of…

A: An artificial nutrient medium contains nutritional components like sugars, salts, amino acids, etc.…

Q: What is meant by the term plating efficiency?

A: The cells are cultured over the plate at the particular growth conditions within a particular time…

Q: For an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h,…

A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…

Q: A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it.…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Q: CFU/mL

A: in  calculation via conventional formula , CFUs/ml in 2nd dilution are greater than 1st dilution…

Q: cal is utilized as the reagent known as VP-B?

A: The Voges-Proskauer (VP) test is generally used to test if an organism produces acetyl methyl…

Q: How much liquid media should you put in your dilution blank if you wanted to serially dilute a1 ml…

A: Serial dilution of the original sample containing microorganisms to achieve countable colonies on…

Q: Aside from Paraffin, what is your recommended infiltrating medium for routine purposes? why?

A: INFILTRATION is the process in which the clearing agent is completely removed from the tissue and…

Q: 1) Write your dilutions as a rational number AND in scientific format? What was your total dilution…

A: Serial dilution is a process in which a sample is taken and diluted through a series of standard…

Q: What are the advantages of granulation ? during tablet formulation.

A: Introduction: A tablet formulation commonly contains at least one dynamic substance and other…

Q: You dilute 1 mL of bacterial culture with 99 mL of water.  What is the resulting dilution factor?

A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…

Q: What is diluent? Why is it useful for working with bacterial samples?

A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…

Q: If the seven-digit profile that is tabulated cannot be found in the API® 20E Analytical Profile…

A: The API® 20E System, is a biochemical panel for identifying and differentiating members of the…

Q: What is the basic purpose of the paper chromatography?

A: Chromatography is an important analytical method used to separate a mixture of chemical substances…

Q: Please explain why is 70% ethanol used as an effective disinfecting solution in labs? Why not 95%…

A: Only 70% alcohol is effective as a disinfectant that is killing the microorganism on the surfaces.…