Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a hospitalized patient with a urinary tract infection. The organism produced colorless colonies on MacConkey agar and gives the following
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- Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.This is a catalase-positive, coagulase-negative, gram-positive coccus isolated from a urine specimen from a 20-year-old female college student. The image shown is a Mueller Hinton plate streaked with a 0.5 MacFarland standardized inoculum and a 5 microgram disk of novobiocin after overnight incubation. What is the identification of the isolate? Please select the single best answer Staphylococcus epidermidis Staphylococcus aureus Staphylococcus saprophyticus Staphylococcus lugdenensisname the genus and species of 2 enteric bacteria that do not ferment lactose discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.
- Why does the oxidase test must be performed from blood agar or another medium without a fermentable sugar as MacConkey agar?What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is the reagent used in this test? What media is used? Describe the biochemical abilities of these organisms .What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What is the media used? What is the product of this test that reacts with the reagent to give positive result? What biochemical abilities do these organisms have (or lack)?
- Why is CFU more applicable to a culture of Streptococcus than to a culture of E. coli?Erysipelothrix rhusiopathiae is a gram positive bacilli that survives in water contaminated by a sick animal’s feces or urine, soil, or plant material. It is known for causing zoonoses, and is typically associated with occupational exposure to animals. There are a few tests commonly used that help to differentiate the organism. Indicate the results for the following reactions: Catalase: TSI: Growth in gelatin:Does Escherichia coli provide positive or negative results for the lysine decarboxylase and ornithine decarboxylase test results?
- Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when performing the oxidase test in microbiology lab?Between Bacillus megaterium, Bacillus sphaericus, and Bacillus subtilis which are positve and which are negative for oxidase test results?What is the dilution factor used for the glycogen isolate? Show complete solution.