if 250 colonies are present on a pour plate made from10^5 dilution, how many colonies should grow on a plate made from 1:10^6 dilution? Explain.
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if 250 colonies are present on a pour plate made from10^5 dilution, how many colonies should grow on a plate made from 1:10^6 dilution? Explain.
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- What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?1. if 250 colonies are present on a pour plate made from 1:10^5 dilution, how many colonies should grow on a plate made from 1:10^6 dilution? Explain? 2. list two additional factors that contribute to error in the pour plate method:given a phage lysate with 10^11 pfu/ml and a bacterial culture with 10^8 cfu/ml, how would you obtain MOI =0.1 ?
- If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramWhat side of the electrophoresis chamber (red or black) should the wells be on when the gel is placed in the chamber? What would happen if you placed the agarose gel with the wells on the opposite side of the chamber?How would you prepare 10 to the power -4 dilution from a culture?
- In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?For an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h, calculate g, n, and k forthis culture.You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor?
- Why is the colony diameter of the yeast culture grown on potato dextrose agar is larger than those grown on acetate agar? Give and discuss the reasons for this different in growth.How many microliters of the 500mM colchicine is required for making 1.5mL cell culture with 2mM colchione?The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?