If the length of E.coli DNA is 1.36 mm,can you calculate the number of base pairs in ECOLI?

Q: How is the melting curve of duplex DNA aff ected by adding a small amount of ethanol?

A: Melting curve analysis is an assessment of the dissociation characteristics of double-stranded DNA…

Q: In gel electrophoresis, are the DNA fragments attracted to, or repelled from the negative pole of…

A: Electrophoresis is a process where charged particles move under the influence of the electric field.…

Q: List three rules for designing primers?

A: A primer is short ,single strand DNA sequence used in the polymerase chain reaction. Primers are…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: What is a Watson–Crick base pair?

A: Two nucleotides in the complementary strands of the nucleic acids are connected to each other by…

Q: If DNA concentration is 2 grams, what would be the molar concentration of human DNA in a human cell?

A: Molar concentration is sometimes referred to as molarity, concentrations of an amount, or content of…

Q: When proteins are separated using native gel electrophoresis, size, shape, and charge control their…

A: Gel electrophoresis is a method for separation and analysis of macromolecules like DNA, RNA, and…

Q: Which nucleotides break the rules of Watson–Crick basepairing when they are found at the wobble…

A: The wobble hypothesis states that the base at 5' end of the anticodon is not spatially confined as…

Q: Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move…

A: Electrophoresis the separation of fragment based on their size and charge.Agarose gel…

Q: Given the following mismatch (highlighted in red), which base will be replaced after mismatch repair…

A: Normal metabolic activities and environmental exposure can cause DNA damage. DNA damage in human…

Q: For a linear B-DNA molecule of 50,000 kb, calculate (a) the contour length and (b) the length of the…

A: The complexity of an organism is based on the functioning of its genome. Deoxyribonucleic acid (DNA)…

Q: Approximately how large is the Escherichia coli genome in basepairs? How many genes does it contain?

A: Escherichia coli also known as E. coli is a gram negative, facultative anaerobic, rod shaped,…

Q: What is the axial ratio (length:diameter) of a DNA molecule 20 μm long?

A: Deoxyribonucleic acid is a molecule composed of two polynucleotide chains that coil around each…

Q: How many DNA bands would you observe in an ethidium bromide-stained electrophoresis gel if you…

A: In agarose gels and various cesium chloride gra- dient techniques, ethidium bromide is employed to…

Q: Extracting DNA From Strawberries Is isopropyl alcohol polar or nonpolar? Explain

A: DNA extraction is used to isolate dna molecule from the cells . It helps in removing impurities and…

Q: How often, on average, will the endonuclease AluI, which cleaves the sequence 5’ AGCT 3’, cut normal…

A: Enzyme restriction, also referred to as endonuclease restriction, is a protein formed by bacteria…

Q: consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5'. if the given DNA sample was…

A: Ion Torrent sequencing is also known by the name of: Ion semiconductor sequencing pH-mediated…

Q: If hypoxanthine (structure shown) was incorporated into double stranded DNA, what would it most…

A: Hypoxanthine is a non-natural base that is found in the DNA. DNA is also known as deoxyribonucleic…

Q: When you isolate DNA, what is the purpose of the hot water and the detergent in the activity?

A: The basic principle related to DNA isolation is to distrup the cell wall, cell membrane and nuclear…

Q: The DNA of a deletion of alpha bacteriophage has a length of 15 micrometers instead of 17…

A: The bacteriophage is those viruses that have the capability to infect bacteria and reproduce inside…

Q: Consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5' If the given DNA sample was…

A: Ion torrent sequencing : The ion torrent sequencing techniques makes use of the principle that when…

Q: A 1% by weight agarose gel is typically used in agarose gel electrophoresis. If you used a 2%…

A: During agarose gel electrophoresis, an agarose solution is prepared, it is cooled to prepare a gel,…

Q: When a segment of DNA containing either aVNTR or an RFLP is analyzed, the result is fragments of DNA…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: The E. coli genome contains 1009 Chi sequences. Do these sequences occur at random, and, if not, how…

A: Recombination is the process of genetic reshuffling where the genetic material is shared within or…

Q: IT IS POSSIBLE TO ESTIMATE THE CONCENTRATION OF SPECIFIC DNA SEQUENCE IN A SOLUTION OF MIXED DNA.…

A: DNA can be quantified through nanodrop with a particular absorbance.

Q: Is it possible to obtain DNA in cloverleaf configuration? Explain

A: Cloverleaf Model:  The cloverleaf model of tRNA is a representation of the molecule's structural…

Q: Why does evenly distributed peak in DNA chromatogram is an indication of a good sequence?

A: Evenly spaced peaks indeed indicate a good DNA sequence. Generally, four colours of peaks are…

Q: What would be a primer sequence synthesized from the following DNA sequence: 5'-ACGTG-3'?

A: In the polymerase chain reaction (PCR), a primer is a short, single-stranded DNA sequence. A pair of…

Q: In this picture of gel electrophoresis of DNA, which sample contains the longest/largest piece of…

A: DNA (Deoxyribonucleic acid) is a double helical structure that consists of nucleotide. Nucleotide…

Q: What are the consequences for a DNA sequencing reaction if the ratio of dideoxyribonucleoside…

A: Introduction  DNA is composed of nucleotides arranged in a specific sequence. Prior knowledge of DNA…

Q: What is the concentration of a DNA solution that absorbs 0.812 and 0.463 at 260 and 280 nm,…

A: Deoxyribonucleic acid (DNA) is the genetic material. It is polymer of nucleotides. The protein…

Q: For a closed-circular DNA molecule of 6,825 base pairs in the fully relaxed form, the linking number…

A: In simple words, the linking number represents the number of times a curve winds around another. The…

Q: Base analysis of a plant DNA shows it to have 15 mole percent adenine (moles per 100 moles total…

A: DNA (Deoxyribonucleic Acid) It is defined as a genetic material that has all the stored genetic…

Q: What is the purpose of the dideoxynucleotide triphosphates in the Sanger sequencing reaction?

A: In 1977, Sanger proposed chain termination method of DNA sequencing. He used ddNTP or…

Q: Supercoiled DNA migrates through an agorose gel faster than similarly sized linear DNA? True or…

A: According to bartleby guidelines, when multiple questions are posted. We are allowed to answer the…

Q: During agarose gel electrophoresis, why does DNA move through the gel when electric current is…

A: Gel electrophoresis is basically a method that aid in separating DNA fragments and other molecules…

Q: BamHI cut sequence: G//GATCC and each sequence is 250 nucleotides long. How many DNA segments would…

A:

Q: What is a DNA library? Do you think this name is appropriate?

A: In terms of molecular biology, a library can be defined as the collection of DNA fragments that are…

Q: From the above sequence (DNA: ATGGATCCGCTTTAG), what is the amino acid sequence?

A: - Cells decrypt mRNAs by reading their nucleotides in teams of 3, known as codons. Here square…

Q: If the length of E. coli DNA is 1.36 mm, can you calculate the number of base pairs in E.coli?

A: DNA is the genetic material in E.coli. It has a circular DNA molecule 4.6 million base pairs in…

Q: Describe the various characteristics of the Watson–Crick doublehelix model for DNA.

A: The hereditary ingredient in the cell is genetic material. It contains all of an organism's…

Q: Are the following base sequences sticky or not sticky? Each piece is written 5′ to 3′.(a) TTAGC and…

A: A base is nitrogen containing heterocyclic compound which, is found in DNA and RNA.  There are…

Q: Given the Percentage Composition of One Nucleotide ina Genome, Can We Predict the Percentages of the…

A: Nucleotides are the chemical compounds that constitute the nucleic acids. The nucleic acids are of…

Q: If the length of E.coli DNA is 1.36 mm, calculate the number of base pairs it contains

A: Introduction: DNA (deoxyribonucleic acid) is the molecule that transmits genetic information for an…

Q: Convert each of the following 3′-to-5′ DNA base sequences to 5′-to-3′ DNA base sequences. a. 3′…

A: Introduction DNA is a directional molecule because each of its building block (nucleotides) have 5'…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.