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Asked Sep 18, 2019
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If you find E. coli growing in the presence of ampicillin, what are we assuming about where those
9.
resistance genes came from? What other genes are we then assuming that the E. coli now have?
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If you find E. coli growing in the presence of ampicillin, what are we assuming about where those 9. resistance genes came from? What other genes are we then assuming that the E. coli now have?

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Step 1

Escherichia coli (E. coli) is one of the main causes of nosocomial infections in humans. It is usually found in human guts and it’s considered an indicator of fecal contamination in water and food. Pathogenic variants cause intestinal infections, including meningitis, gastroenteritis, urinary tract infection, etc. The widespread use of antibiotics could be associated with the selection of antibiotic resistance mechanisms in pathogenic and non-pathogenic isolates of e.coli.

Genes are the code which transfers from parents to offspring this is called vertical gene transfer wherein bacteria and some lower eukaryotes are unique in that they can pass DNA from one cell of the same generation to another this is called horizontal gene transfer.

Step 2

The three ways for bacteria to transfer their DNA horizontally:

  • Conjunction

The Conjunction is processing in which DNA directly transfer from one cell to another through cell-cell contact. The DNA transfer by conjugation often involves plasmids. The conjugative transfer of plasmids is carried by cell surface structures like syringes, injecting the plasmid into neighboring cells.

  • Transformation

Bacter...

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