Answered: If you place a cuvette with an unknown…

If you place a cuvette with an unknown concentration of that solute in the spectrophotometer and it reads an absorbance of 0.15 AU estimate the concentration of solute inside

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter19: Biomedical Laboratory Instrumentation

Section: Chapter Questions

Problem 10Q

See similar textbooks

Related questions

Q: Which of the following results in green output in spectrophotometry experiment? a. Violet light b.…

A: Spectrophotometry: it is used for quantitative analysis of spectra of various wavelengths to compare…

Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.

A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…

Q: If 5X instead of 10X oculars were used with the same objectives now on your microscope, what…

A: Microscopy means the use of microscope for studying the cells. Microscope is an indispensable…

Q: If we wanted to know how “red” a red liquid solution is, we would use a light transmittance…

A: Medical instrumentation is branch of science which deals with the study of instruments which are…

Q: explain the results of this experiment Table 1 Absorption Spectrum of Cobalt Chloride Using a…

A: We know that the calibration curve can be used to determine the concentration of an unknown…

Q: You use a spectrophotometer to measure the absorbance of a colored solution and find it to be 1.6.…

A: According to Beer- Lambert law when a monochromatic light passes the homogeneous medium, it reflect,…

Q: This is integral to obtaining the experimental data that lead to the development of this model…

A: Introduction ODE MODEL:- Ordinary differential equations or ODE models are used to model biological…

Q: To establish a standard curve for a BSA standard curve using Bradford, the spectrophotometer…

A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…

Q: Draw and label 3 cell types at low and high power. State the magnification for each one

A: Microscopes enable us to magnify specimens. In simple terms , magnification is an increase in size…

Q: WHAT ARE THE Steps in spectrophotometer

A: -Spectrophotometer is an optical instrument which is used to measure the concentration of a known…

Q: Suppose you were in a research lab in Romania and the lab is equipped with several…

A: Proteins are biomolecules that play a variety of roles in living systems. They include catalysis,…

Q: What’s the total magnification for 5x and 44x in a microscope

A: When the size of the image is compared to that of the object that created the image in the first…

Q: Which of these are used to measure optical activity?a) Polarimeterb) Planometerc) Psychrometerd)…

A: The optical activity is the process of the rotation of the polarization plane of a light beam when…

Q: Viewing Object Through the Microscope: If the diameter of the low-power field is 1.5mm, an object…

A: Magnification in a microscope is the ability of the microscope to enlarge the image of the object…

Q: Compare AAS, AES and UV-Vis spectrophotometer

A: The most sophisticated spectrophotometers are AAS, AES and UV-vis spectrophotometers. They each have…

Q: : In x-ray machine, changing the KVP can affect the Energy of the produced x-ray O Intensity of the…

A: KVP stands for kilovoltage peak . This is the highest voltage that will be produced by the X ray…

Q: Microscopic objects often are measured in micrometers. A micrometer equals1/1000 of a millimeter and…

A: Compound microscope showing the 10x ocular (eyepiece) and four objectives (4x, 10x, 40x and 100x).…

Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…

A: An absorption spectrophotometer is defined as a device that is used to calculate the absorbed light…

Q: During observation under the microscope, the number of optical planes that are in focus…

A: Light microscope is a microscopic instrument which uses visible light and magnifying lens to to view…

Q: Can spectrophotometry be used to determine the concentration of “colorless” solutes such as salt or…

A: Spectrophotometry in analytical method for measuring the concentration of a chemical compound in a…

Q: The following is an UV-Vis schematic diagram. Based on this diagram, answer the following questions:…

A: UV-vis spectrophotometer A spectrophotometer is an instrument or apparatus that is used in the…

Q: Let's say that you have a green solution that is made up of a mixture of a yellow chemical and a…

A: Beer Lamberts' law relates the concentration of a solution and the light absorbed by the solution.…

Q: Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2.…

A: Answer: Photometer : It is the instrument which is used to measure the intensity of a beam of light.…

Q: O 1) brightfield O 2) TEM 3) fluorescent O 4) confocal O 5) phase contrast

A: Different microscopes are used in terms of conditions in which we want to visualize the specimen.…

Q: The absorbance of light is measured with a spectrophotometer and the result can be used to estimate…

A: the absorbance of light is measured with a spectrophotometer and result can be used to estimate the…

Q: Enumerate the different kinds of blanks used in spectrophotometry

A: Introduction : Spectrophotometry is a method used to analyse molecules quantitatively based on how…

Q: Use the lens magnification values in the table below and the sample cell (viewed under low power)…

A: 1) total magnification of microscope can be calculated by using formula- Total magnification =…

Q: An object is 20µm in diameter. If viewed through a 10X ocular lens and a 40X objective lens, how…

A: Answer: MICROSCOPY : It is a technique for making small things to visible by magnifying through…

Q: In a spectrophotometer, what should be the appearance on the graph if the sample is pure? presence…

A: Spectrophometry is a widely used technique in identification and confirmation of chemical…

Q: Discuss the concept of spectrophotometry, colorimetery, and turbidimetry. Maximum of 150 words

A: Spectrophotometry is a part of electromagnetic spectroscopy worried about the quantitative…

Q: The ratio of the wavelength in the .1 vacuum to the wavelength within the material is called •…

A: Refractive index. Refractive index is a measure of how light propagates threw a material. Refractive…

Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…

A: Magnification on a microscope denotes the amount or degree of visual enlargement of an observed…

Q: principle of basic spectrophotometer

A: Spectrophotometer is an advanced instument which measures the amount of light absorbed by a sample.…

Q: ssume that you have an “X” solution that you do not know its concentration. But you have other X…

A: Absorbance of X solution Concentration Absorbance 0.025 0.22 0.05 0.44 0.1…

Q: In the spectrophotometer, why is there a need to measure a 'blank' sample? What will happen to the…

A: A spectrophotometer is one of the most frequent tools used by scientists to determine the presence…

Q: spectrophotometer converts light energy into electrical energy?

A: ANS-1 Option B Explanation- Visible light is the only light our eyes can see. Our eyes detect these…

Q: when viewing an organism under the 40x objective lens, you estimate that 6 organims could fit across…

A: 40x objective lens are high powered objective lens which is used to observe fine details in a…

Q: Describe how you would prepare the following samples for analysis by a spectroscopic method. You…

A: Spectroscopic method or techniques is the process that recruit the light to link with matter. This…

Q: spectrophotometry, what is the role of the green background in the set-up? What does it represent in…

A: Introduction:- Spectrophotometry is a technique for determining how much light a chemical substance…

Q: Assume that you have an “X" solution that you do not know its concentration. But you have other X…

A: The spectrophotometric methods is used to measure the absorbance of a sample by measuring the…

Q: What is the magnification of the following drawing of a specimen extending one- hird of the medium…

A: Medium power in a computer microscope has 10X magnification. Size visible = 1/3rd of 1.8mm…

Q: Explain the working principle of Atomic Absorption Spectrophotometer and Draw the general Diagram of…

A: Atomic absorption spectrometery is highly practiced to analyse compounds in the solutions and this…

Q: a. The scale of a spectrophotometer extends from 1 to 100% T, what are the values of these two…

A: Beer- Lambert Law: It is also known as Beer's Law. It states that the intensity of a monochromatic…

Q: Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X…

A: Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X…

Q: A solution containing 2g/liter of a light-absorbance substance in 1 cm cuvette transmits 75% of…

A: Introduction Using the Beer-Lambert law, logI∘I=A=aclwhere, I∘ is the Incident light, I is the…

Q: In a tabular form, enumerate the different components of a spectrophotometer and explain its…

A: A spectrophotometer is an analytical equipment that is used to measure the absorbance of light by…

Q: Which molecules, or function groups, or complexes that may be responsible for the absorbance at 595…

A: Spectrophotometry applications are useful to measure the absorbance, reflectance, and transmission…

Q: Discuss the types of interferences in atomic absorption spectrophotometry.

A: Discuss the types of interferences in atomic absorption spectrophotometry. Introduction: Spectral…

Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?

A: Spectroscopy is a procedure utilized for the evaluation, portrayal, and basic investigation of a…

Q: Which of the following has the shortest wavelenght? A. Radiowave B. infrared c. visible light d.…

A: The question asks about the correct option for having the shortest wavelength.A). Radio-waves B).…

Concept explainers

Microscopy

Microscopy is a technical field of study about the use of microscopes to observe small objects like cells and micro-organisms that are not visible to the naked eye. A microscope is used in the field of microscopy, in order to see the object or organism that cannot be seen easily. Microscopes help us to observe the fine structure of a cell and its content.

Question

If you place a cuvette with an unknown concentration of that solute in the spectrophotometer and it reads an absorbance of 0.15 AU estimate the concentration of solute inside

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step 3

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

a. The scale of a spectrophotometer extends from 1 to 100% T, what are the values of these two extremes in absorbance? b. Convert the 98%T to absorbance
With regard to the spectrophotometer, which of the following is true for regarding the term absorbance? I. The term refers to the amount of light that is prevented from passing through a solution. II. As solute concentration increases the absorbance reading also increases. III. Absorbance is the opposite of transmittance.
Suppose you were in a research lab in Romania and the lab is equipped with several spectrophotometers (UV and VIS). You need to determine the concentration of a protein solution and although you know several techniques you must decide which spectrophotometer to use. Which spectrophotometer are you choosing and which wavelength will you use?
the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.
Which spectrophotometer mode should you use to calibrate the instrument and which mode should you use to read your samples? Transmittance for both Absorbance for both Absorbance to calibrate and transmittance to read samples Transmittance to calibrate and absorbance to read samples Flip a coin to pick a mode each time.
Which of the following results in green output in spectrophotometry experiment? a. Violet light b. Blue light c. Green light d. Yellow light
if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?
If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints, and dust prior to reading it in the spectrophotometer, how might the absorbance value of the sample be affected? (Higher, Lower, No Change) Explain
Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.
Describe how you would use a compound light microscope with the 4 objective lenses to view a specimen on a pre-made slide up to a total magnification of 400X.
A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.
In the spectrophotometer, why is there a need to measure a 'blank' sample? What will happen to the accuracy of your result if you measure the specimens without measuring a 'blank' trial? In the experiment, what liquid can be placed in the cuvette when measuring a 'blank' trial?