In HPLC, why is purging or initialization being done before analysis of samples, explain.

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter33: Automated Methods Of Analysis
Section: Chapter Questions
Problem 33.5QAP: Give the advantages and disadvantages of sequential injection analyzers compared to traditional flow...
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In HPLC, why is purging or initialization being done before analysis of samples, explain. 

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