In order to understand the concepts of pH and buffers, gain experience in using the Henderson-Hasselbalch equation, and to make the buffers you will be using this semester, you will make: 50.0 mL of 10X P, Buffer: 50.0 ml of 25X TE Buffer: 200 mM phosphate at pH 5.6 250 mM Tris with 25 mM EDTA at pH 7.5 The phosphate buffer will be prepared using a solid monobasic phosphate salt, and a solid dibasic phosphate salt. The same pK, works for either the Na* or K* salts. These salts are frequently hydrates, so the specific molar mass is provided.

In order to understand the concepts of pH and buffers, gain experience in using the Henderson-Hasselbalch equation, and to make the buffers you will be using this semester, you will make: 50.0 mL of 10X P, Buffer: 50.0 ml of 25X TE Buffer: 200 mM phosphate at pH 5.6 250 mM Tris with 25 mM EDTA at pH 7.5 The phosphate buffer will be prepared using a solid monobasic phosphate salt, and a solid dibasic phosphate salt. The same pK, works for either the Na* or K* salts. These salts are frequently hydrates, so the specific molar mass is provided.

Chemistry: Principles and Practice

3rd Edition

ISBN:9780534420123

Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer

Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer

Chapter16: Reactions Between Acids And Bases

Section: Chapter Questions

Problem 16.7QE

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