In reverse phase chromatography which analyte will elute first, most polar or least polar?

Q: How chromatogram is developed in thin layer chromatography?

A: Chromatography is a technique used to separate mixture of substances into their components. It…

Q: economical purification technique that ca n be pure material? O Distillation O Another extraction…

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Q: two general methods for improving the resolition of two  substances on chromatography column

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Q: In Thin Layer Chromatography，why does the least polar of the analytes （biphenyl）have a higher Rf…

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Q: In your understanding, what is meant by reversed-phase chromatography? Explain its difference from…

A: Reversed phase chromatography is a technique in chromatography that is developed recently.  This is…

Q: Capillary electrophoresis differs from other chromatographic methods because:

A: It uses unique detectors.

Q: The eluant in column chromatography is typically referred to as: 1) the stationary phase 2) the…

A: Chromatography is a technique in which we separate a single chemical compound from a mixture. If the…

Q: Which is the correct order of elution of a gas chromatography that has a polar column that contains…

A: The sample, which may be a gas or a liquid, is injected into a gaseous mobile phase during gas…

Q: Retention Time Of Cyclohexene?

A: Retention time is the time interval between compound introduced or chromatogram starting to maximum…

Q: The chromatogram indicates that O MGDG eluted first from the column DGDG SGDG

A: The given chromatogram indicates the retention time of three components.

Q: what is the best choice of detector for lorazepam in Gas chromatography

A: The best choice of detector for lorazepam in Gas chromatography is nitrogen-phosphorus…

Q: Explain the relationship between polarity of the compound and retention factor in chromatography

A: In chromatography, retention factor is the ratio of the distance travelled by the individual…

Q: Is it possible to detect metal complexes of organic acids with thin layer chromatography?

A: Thin layer chromatography, abbreviated as TLC, is used to identify the element or compound present…

Q: Why do we need to add sand on top of the silica in column chromatography? What could happen if we…

A: The presence of different compounds in the mixture is separated by employing the separation methods.…

Q: In chromatography, if the temperature of oven increases, what will be its effect on retention time…

A: Chromatography It is the separation and purification technique used in which a stationary phase and…

Q: Using a normal phase chromatography, which solute will elute out first from column? Chlorophyll…

A: Answer In column chromatography the more polar compounds…

Q: Why is it important to mark the solvent level on the chromatography paper as soon as you remove it…

A: We know that, it is important to mark the solvent level on the chromatography paper as soon as it is…

Q: How can you use ion-exchange chromatography to de-ionize water?

A: How can you use ion-exchange chromatography to de-ionize water?

Q: paper chromatography?

A: Chromatography is a technique that is used for the separation of a mixture. It has two important…

Q: Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse…

A: Reversed  phase chromatography  have  long chain alkyl chains which are non polar in nature .

Q: Differentiate between gel filtration and gel permeation chromatography.

A: Given : We have to tell what is the difference between gel filtration and gel permeation method.

Q: What is meant by retention time in gas chromatography.  In what order will they come hexane, ethyl…

A: The process of separation of various constituent of a mixture in zone or in phases is known as…

Q: In thin-layer chromatography, what will be the result if the solvent level is higher than the sample…

A: A multiple choice question based on thin layer chromatography, which is to be accomplished.

Q: What is reverse-phase ion-pairing chromatography?

A: This is an more efficient Technique in which counter ions of solute particles are used .

Q: a pigment in the thin layer chromatography traveled beyond or farther than the solvent front.…

A: As a pigment in the thin layer chromatography traveled beyond or farther the solvent front therefore…

Q: An orange compound was added to the top of a chromatography column. Solvent was added immediately,…

A: An orange compound was added to the top of a chromatography column. The solvent was added…

Q: would column chromatography separate ketone and alcohol

A: The detailed solution of the given question is solved below.  COLUMN CHROMATOGRAPHY  Column…

Q: Why do we use a makeup gas for some gas chromatography detectors?

A: The method of separation of components present in the mixture is termed as chromatography.…

Q: 1. In thin layer chromatography (TLC), why do you need to use a pencil in marking the spots, origin,…

A: 1. Marker spot is soluble in the solvent. Therefore it will move on TLC plate with solvent. 2.…

Q: applications

A: Let us discuss the principle of size exclusion chromatography: Here the selection of molecules is…

Q: In one paper chromatography, the Rf for spots X and Y are 0.5 and 0.35 respectively. The solvent…

A: In paper chromatography Rf value is the ratio of distance travelled by compound to distance…

Q: What is the difference between an ascending and descending paper chromatography?

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Q: n paper chromatography,

A: Paper chromatography was discovered by Synge and Martin in the year 1943.

Q: What do the separator and suppressor do in ion chromatography?

A: Ion chromatography is also termed as ion exchange chromatography. Ion chromatography is used for the…

Q: In normal phase chromatography, water is a weaker eluent than methanol., True or False?

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Q: 24. In paper chromatography, a rectangular piece of filter paper serves as the 24. A. mobile phase…

A: Paper chromatography was discovered by Synge and Martin in the year 1943. In paper chromatography,…

Q: Can acetic acid be used as the mobile phase in  kool aid chromatography over isopropyl alcohol?

A: Given Can acetic acid be used as the mobile phase in  kool aid chromatography over isopropyl alcohol

Q: What is Jon chromatographys

A: Given To know about the chromatography

Q: "Start the chromatography by adjusting the fraction collector to 130 drops per fraction (about 3…

A: It is given that for 130 drops per fraction it shows volume 3 mL That means volume of 130 drops is 3…

Q: What is an evaporative-light scattering detector in chromatography?

A: An evaporative light scattering detector (ELSD) is a detector used in conjunction with…

Q: Match column A with Column B. Everything is all about column Chromatography v Least polar molecules…

A: This question is related to column chromatography. Column chromatography is a technique used to…

Q: 7. Which of the following compounds will elute LAST in a reverse phase chromatography? A. ONF

A: In reverse phase chromatography, the stationary phase is non-polar and mobile phase is polar.

Q: Determine the order of inhibition time of hexane, hexanol and benzene compounds in the reverse phase…

A: Given compounds: Hexane, hexanol and Benzene. To determine: The order of Inhibition time in Reverse…

Q: How GC retention time can be affected by the mobile phase flow rate and the temperature of the…

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Q: Why do brands broaden as they travel through a chromatography column ?

A: In analytical chemistry, To separate a single compound from a mixture, Column chromatography is…

Q: thin layer chromatography What would happen to it if you decreased the polarity of the mobile…

A: Thin Layer Chromatography is used to monitor the progress of the reaction. It is composed of the…

Q: 35. The stationary phase, as well as mobile phase, are liquid in Adsorption column chromatography…

A: Answer: Chromatography is a separation technique, in which we separate the components of a mixture…

Q: What type of materials can be used as the stationary phase for column chromatography? O Inorganic…

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