In terms of counting cells, what does a plating method accomplish that an electronic cell counting method does not?

10. Which area on a streak plate will contain the greatest amount of growth? The least amount of growth? Explain your answers. 11. What is a colony (as viewed on an agar plate)? How can a pure culture be obtained from a mixture of bacteria? 12. What is a selective agar? Why can it be of importance?

So is it fair to say that  #1 treatment 1 has steady growth  treatment 2 has stead growth  treatment 3 has steady growth treatment 4 has very little growth treatment 5 has some growth treatment 6 steady growth #2 The carbon dioxide provided a good amount of growth

4. You used 1mL of a river water sample at a 10-3 dilution and added this to an agar plate. After incubation, you count 60 colonies. How many colony forming units (CFUs) are present? Show your work.

29.  Identify a FALSE statement from the following,  Group of answer choices bacterial growth is related to nutrient concentration and availability sulfur is essential in growth medium for some amino acid synthesis none of the above is an false statement nutrients uptake can be via active or passive process culture medium must be sterile before inoculation     30.  Identify a CORRECT statement regarding  penicillin allergy? Group of answer choices the hypersensitive reactions are mediated by IgE penicillin is degraded to penicilloyl and other breakdown products all of the statements are correct penicilloyl haptens trigger most allergic reactions anaphylactic shock is the immediate danger penicillin can provoke

2. a. explain 5 methods of nutrient uptake by cells b. explain the problems that may arise in the process of media sterilization using heat

5. Give two reasons for why heat fixing is used?  Adherence of bacterial cells to the slide Denaturing of bacterial enzymes to preserve cellular integrity

5. Using a pencil as your loop draw the streaking pattern used on a Petri plate to best achieve isolated colonies. How many times do you dip your loop into a broth culture? Number each streak in order.

1. how does colony appearance and location with respect to the agar differ on the spread and pour plate? 2. list two additional factors that contribute to error in the spread plate method:

1. Which of the following methods is the best way to sterilize the apparatus used in a surgical operation? A. Pasteurization B. Incineration C. Boiling in water D. Autoclaving E. Disinfect with 75% alcohol