Justify your answer with calculations of partial charges on these amino acid side chains at pH = 7.8. (Hint: Consider lessons from Coulomb's law, and the Henderson-Hasselbalch equation.) Express your answers using two significant figures separated by a comma. Π| ΑΣΦ - 0.99,0.04 Submit Previous Answers Request Answer ? Incorrect: Try Again: 3 attempts remaining

Fundamentals Of Analytical Chemistry
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Chapter14: Principles Of Neutralization Titrations
Section: Chapter Questions
Problem 14.32QAP
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answer part C/last question only. box the answer and MAKE SURE IT IS CORRECT

Justify your answer with calculations of partial charges on these amino acid side chains at pH = 7.8. (Hint: Consider lessons from Coulomb's law, and the Henderson-Hasselbalch equation.)
Express your answers using two significant figures separated by a comma.
ΑΣΦ
- 0.99.0.04
Submit
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Transcribed Image Text:Justify your answer with calculations of partial charges on these amino acid side chains at pH = 7.8. (Hint: Consider lessons from Coulomb's law, and the Henderson-Hasselbalch equation.) Express your answers using two significant figures separated by a comma. ΑΣΦ - 0.99.0.04 Submit www. ]] ? Previous Answers Request Answer Incorrect: Try Again: 3 attempts remaining
Part A
Do you predict that this salt bridge will become stronger, become weaker, or be unaffected as pH increases from
pH = 7.2 to pH = 7.8?
O The salt bridge will become stronger.
The salt bridge will become weaker.
The salt bridge will be unaffected.
Submit
✓ Correct
At pH = 7.2 the glutamic acid (Glu) side chain will carry a charge of ~ -1 (at 3 pH units above the pK₂ for
Glu, the side chain will be almost fully ionized); whereas the histidine (His) side chain will carry a charge of <
+0.5 (at pH=pKa the charge on His would be +0.5; since pH = 7.2 is above its pKa, it will carry less (+)
charge as it becomes more deprotonated). As the pH increase to 7.8, the charge on Glu will remain ~ -1 and
the charge on His will decrease; thus, this salt bridge is predicted to become weaker as pH is raised from 7.2
to 7.8.
Part B
Previous Answers
Justify your answer with calculations of partial charges on these amino acid side chains at pH = 7.2. (Hint: Consider
lessons from Coulomb's law, and the Henderson-Hasselbalch equation.)
Express your answers using two significant figures separated by a comma.
-1.0,0.17
Submit
Previous Answers
Transcribed Image Text:Part A Do you predict that this salt bridge will become stronger, become weaker, or be unaffected as pH increases from pH = 7.2 to pH = 7.8? O The salt bridge will become stronger. The salt bridge will become weaker. The salt bridge will be unaffected. Submit ✓ Correct At pH = 7.2 the glutamic acid (Glu) side chain will carry a charge of ~ -1 (at 3 pH units above the pK₂ for Glu, the side chain will be almost fully ionized); whereas the histidine (His) side chain will carry a charge of < +0.5 (at pH=pKa the charge on His would be +0.5; since pH = 7.2 is above its pKa, it will carry less (+) charge as it becomes more deprotonated). As the pH increase to 7.8, the charge on Glu will remain ~ -1 and the charge on His will decrease; thus, this salt bridge is predicted to become weaker as pH is raised from 7.2 to 7.8. Part B Previous Answers Justify your answer with calculations of partial charges on these amino acid side chains at pH = 7.2. (Hint: Consider lessons from Coulomb's law, and the Henderson-Hasselbalch equation.) Express your answers using two significant figures separated by a comma. -1.0,0.17 Submit Previous Answers
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