2. Make a viable count of the donor culture by spot inoculation ofthe nutrient agar plate WITHOUT antibiotics as follows:• Prepare a 10 fold serial dilution (to 10-7) of the DONOR culture insterile saline.• Show clearly how you intend to prepare this dilution series andhave it checked by a demonstrator before continuing.DilutionVolume ofsaline (units?)Volume of10-110-210-310410-510-610-7which other| dilution?• Clearly label the base of the nutrient broth agar plate as shown inthe diagram below. Include your initials, the date, and what theplate contains (donor viable cell count).• Carefully dispense 3 x 10 µl drops of the 104, 10-5, 106 and 10-7dilutions of the culture within quarter areas of the agar plate thenreplace the lid. Allow the inocula to soak into the agar beforeinverting the plate for incubation.• What is the purpose of this step?49

Nutrient agar is generally preapared for the bacterial growth to carry out biochemical reations on…

Make a viable count of the donor culture by spot inoculation of he nutrient agar plate WITHOUT antibiotics as follows: Prepare a 10 fold serial dilution (to 107) of the DONOR culture in sterile saline. Show clearly how you intend to prepare this dilution series and have it checked by a demonstrator before continuing. Dilution Volume of saline (units?) Volume of which other 10-1 10-2 10-3 104 10-5 10-6 10-7 dilution? Clearly label the base of the nutrient broth agar plate as shown in

Make a viable count of the donor culture by spot inoculation of he nutrient agar plate WITHOUT antibiotics as follows: Prepare a 10 fold serial dilution (to 107) of the DONOR culture in sterile saline. Show clearly how you intend to prepare this dilution series and have it checked by a demonstrator before continuing. Dilution Volume of saline (units?) Volume of which other 10-1 10-2 10-3 104 10-5 10-6 10-7 dilution? Clearly label the base of the nutrient broth agar plate as shown in

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.3: Principles Of Chemistry Instrumentation

Problem 1CT

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