Most yeast grow suspended in culture, but some yeast grow as a film on the top of a liquid culture. These are called flor yeast. The following Southern blot is looking at the FL011 gene that is thought to be involved in whether yeast are flor or not. DNA was isolated from four different yeast strains. Strains 1 and 3 are normal while strains 2 and 4 are flor (Fidalgo, 2006). Based on the gene map and Southern results below, what aspect of FLO11 determines whether a strain will be flor or not? Hincll Hinell TANDEM REPEATS Hindl!! ORF PEOn 23 Kb dAB) 3е кь OR 1.5 Kb 23.1 Kb 94 Кь- 6.5 Kb- 4.3 Kb 3.0 Kb 2.3 Kb 2.3 Kb 20 Kb 1.5 Kb 1 2 3 4 Sh 8-00 12/2/ DELL

Most yeast grow suspended in culture, but some yeast grow as a film on the top of a liquid culture. These are called flor yeast. The following Southern blot is looking at the FL011 gene that is thought to be involved in whether yeast are flor or not. DNA was isolated from four different yeast strains. Strains 1 and 3 are normal while strains 2 and 4 are flor (Fidalgo, 2006). Based on the gene map and Southern results below, what aspect of FLO11 determines whether a strain will be flor or not? Hincll Hinell TANDEM REPEATS Hindl!! ORF PEOn 23 Kb dAB) 3е кь OR 1.5 Kb 23.1 Kb 94 Кь- 6.5 Kb- 4.3 Kb 3.0 Kb 2.3 Kb 2.3 Kb 20 Kb 1.5 Kb 1 2 3 4 Sh 8-00 12/2/ DELL

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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A group of genetics students mix two auxotrophic strains of bacteria: one is leu+ trp+ his− met− and the other is leu− trp− his+ met+. After mixing the two strains, they plate the bacteria on minimal medium and observe a few prototrophic colonies (leu+ trp+ his+ met+). They assume that some gene transfer has taken place between the two strains. How can they determine whether the transfer of genes is due to conjugation, transduction, or transformation?
DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a− b− c− d− e−. Pairs of genes are checked for cotransformation, and the following results are obtained: Pair of genes Cotransformation Pair of genes Cotransformation a+ and b+ No b+ and d+ No a+ and c+ No b+ and e+ Yes a+ and d+ Yes c+ and d+ No a+ and e+ Yes c+ and e+ Yes b+ and c+ Yes d+ and e+ No On the basis of these results, what is the order of the genes on the bacterial chromosome?
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What is the map distance (in minutes) between these two genes?
DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a– b– c– d– e–. Pairs of genes are checked for co-transformation and results shown below are obtained. Based on these results, what is the order of the genes on the bacterial chromosome? please show how you worked out the order by diagraming, thank you! Pair of genes Co-transformation a+ and b+ no a+ and c+ no a+ and d+ yes a+ and e+ yes b+ and c+ yes b+ and d+ no b+ and e+ no c+ and d+ no c+ and e+ yes d+ and e+ no
Recombinant protein production by a genetically modified Escherichia coli strain is proportional to cell growth. Ammonia is used as a nitrogen source for aerobic glucose respiration. The recombinant protein has the general formula CH1,55O0,31N0,25, while that of the cellular biomass is CH1,77O0,49N0,24. The biomass yield from glucose equals 0.50 g/g, while the recombinant protein yield from glucose corresponds to 20% of the cell yield from substrate.a) How much ammonia is required? What is the oxygen demand? (b) If the biomass yield remains the same, what are the ammonia and oxygen requirements for a wild-type strain of E. coli, with cell biomass of the same elemental composition, but unable to synthesize the recombinant protein? (c) On an industrial scale, cultivation takes place in a continuous fermenter at 28°C and the desired recombinant protein production rate is 7 g/h. Since the viscosity of the culture broth is considerable, the energy input due to agitation cannot be neglected.…
You generate several temperature sensitive mutant strains of bacteria. To study what genes might be affected, the mutant strains are cultured along with the normal parental strain at the permissive temperature (37°C) and then shifted at 10 minute intervals to the non permissive temperature (42°C). The only nucleotides in the growth environment are labeled with 32P. The amount of DNA at each step is measured by determining the amount of labeled nucleotides that have been incorporated into the DNA. The numbers refer to the relative amount of labeled nucleotides that have been incorporated. The following results are obtained.(picture) Which strains/strains, if any, has a mutation in a gene required for DNA replication? Explain your experimental predictions and the degree to which the data support/fail to support them.
All of the following pieces of information are expected to be obtained directly from Western Blot analysis, except: An estimation of the fusion protein’s size Relative quantity of the fusion protein compared to other samples The amino acid sequence of the fusion protein If the fusion protein is present in the sample
Three different Hfr donor strains are mixed with separate samples of an F− strain, and the following mapping data are provided from studies of interrupted conjugation: Appearance of genes in F − cells Hfr1: Genes b+ d+ c+ f+ g+ Time* 3 5 16 27 59 Hfr2: Genes e+ f+ c+ d+ b+ Time 6 24 35 46 48 Hfr3: Genes d+ c+ f+ e+ g+ Time 4 15 26 44 58 Construct a genetic map for these genes, indicating their order on the bacterial chromosome and the distances between them.
A bacterial transformation is performed with a donor strain that is resistant to four drugs, A, B, C, and D, and a recipient strain that is sensitive to all four drugs. The resulting recipient cell population is divided and plated onmedia containing various combinations of the drugs. The following table shows the results.a. One of the genes is distant from the other three, which appear to be closely linked. Which is the distant gene? b. What is the likely order of the three closely linked genes
E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interference
Bacteriophage lambda (λ) consists primarily of a head, which contains the genomic DNA, and a tail that is involved in phage attachment to bacterial cells. The diagram to the right represents the bacteriophage lambda genomic DNA, showing the locations of important gene clusters (Ausubel et al. 1998). Arrows below mark the sites where the restriction enzyme HindIII cuts the DNA, and the numbers indicate the number of base pairs in each fragment. The restriction enzyme HindIII cuts the λ genome 7 times, how many fragments are produced following this reaction?
The figure below shows a partial chromosome map of an E. coli Hfr strain. Each mark = 10 minutes between conjugation transfer time. If transfer of genes begins at “*” relative to the origin of transfer, what is one of the predicted results from this map? It would take less than 30 minutes to transfer all of the genes that are shown. gal and azi will rarely be transferred together. gal and ton will rarely be transferred together. Ten minutes after transfer of ton, lac will be transferred. This strain will produce very few gal recombinants.