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Mutations that create auxotrophs unable to grow why?
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- Genetically Modified Foods The creation of transgenic crop plants using recombinant DNA methods involves the transfer of just one gene or a small number of genes to the plants, in contrast to classical breeding methods in which hundreds or even thousands of genes are transferred at once. Explain why this is true. If fewer genes are transferred during the creation of transgenic crops, why are some people afraid that they are dangerous?Why is the use of auxotrophy not a good method for controllingthe growth of a genetically modified organism?In a gene mapping experiment, DNA from a prototrophic E. coli strain is transformed into an E. coli strain mutant (auxotrophic) for the genes gal, pro, and lac. Some resulting transformants were found to be auxotrophic only for pro, and some only for gal, but no lac- only auxotrophs were observed. Based upon these results, which gene is in the middle? a. gal b. lac c. pro d. cannot be determined c. the genes are not on the same chromosome
- "the supplement that restored growth would be the molecule that the mutant strain could not synthesize" Explain this statement ?Scientists who study amino acid biosynthesis pathwayswant to isolate auxotrophic bacteria. A technique calledpenicillin enrichment makes this task easier. This procedure starts by exposing a liquid culture of wild-type (prototrophic) bacteria growing in rich (complete)medium to a chemical mutagen. After this treatment,the cells are centrifuged to remove the liquid and themutagen. The pellet of cells at the bottom of the centrifuge tube is now resuspended in medium that lacksone amino acid (in this example, cysteine) but contains penicillin. Subsequently, the bacteria are pouredonto a filter that concentrates them and allows themto be washed free of the penicillin. The living bacteriaretained on the filter are highly enriched for cysteineauxotrophs.a. Given what you know about the action of pencillin,explain why this enrichment occurs.b. Penicillin enrichment is not a selection, becausethe drug does not kill 100% of the prototrophs.The cells on the filter thus need to be screenedfor…Why golden rice was created? What is its purpose?
