O0000000OTCOCO Procedure: 1. Prepare the gel box, as shown by your instructor. A comb must be suspended in the box and the black walls inserted, otherwise leakage will occur. The comb must be closer to the black electrode. 2. Obtain a tube containing 25 mL of 3 % liquid agarose from the incubator. Pour the liquid gel into the rectangular casting tray. It should fill the tray almost to overflowing. Verify that the tips of the comb are submerged in the gel mixture. (When the comb is removed, indentations called wells will be present, into which DNA samples can be loaded.) The lab technician has prepared liquid gel for you. To make this solution, she first drew up a 3% agarose solution by mixing dry agarose power with buffer solution. Then she heated the agarose solution to dissolve the agarose powder. Our lab tech drew up 1 liter of 3% (g/100 mL) agarose solution. How many grams of agarose powder did she measure out to prepare this solution? How many mL of buffer did she measure out to prepare the solution? 3. Allow at least 15 min for gel to cure (solidify). 4. While the gel cures, practice using the micropipette to load 15 uL samples in the practice gel your instructor has set out for the class. You need to be very careful that you do not puncture the well with the pipet tip when you load samples.
O0000000OTCOCO Procedure: 1. Prepare the gel box, as shown by your instructor. A comb must be suspended in the box and the black walls inserted, otherwise leakage will occur. The comb must be closer to the black electrode. 2. Obtain a tube containing 25 mL of 3 % liquid agarose from the incubator. Pour the liquid gel into the rectangular casting tray. It should fill the tray almost to overflowing. Verify that the tips of the comb are submerged in the gel mixture. (When the comb is removed, indentations called wells will be present, into which DNA samples can be loaded.) The lab technician has prepared liquid gel for you. To make this solution, she first drew up a 3% agarose solution by mixing dry agarose power with buffer solution. Then she heated the agarose solution to dissolve the agarose powder. Our lab tech drew up 1 liter of 3% (g/100 mL) agarose solution. How many grams of agarose powder did she measure out to prepare this solution? How many mL of buffer did she measure out to prepare the solution? 3. Allow at least 15 min for gel to cure (solidify). 4. While the gel cures, practice using the micropipette to load 15 uL samples in the practice gel your instructor has set out for the class. You need to be very careful that you do not puncture the well with the pipet tip when you load samples.
Chapter10: Sample Preparation And Handling
Section: Chapter Questions
Problem 3.1E
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Please answer the questions in the grey colored box provided in the image.
Transcribed Image Text:O0000000OTCOCO
Procedure:
1. Prepare the gel box, as shown by your instructor. A comb must be suspended in the box and
the black walls inserted, otherwise leakage will occur. The comb must be closer to the black
electrode.
2. Obtain a tube containing 25 mL of 3 % liquid agarose from the incubator. Pour the liquid gel
into the rectangular casting tray. It should fill the tray almost to overflowing. Verify that the
tips of the comb are submerged in the gel mixture. (When the comb is removed, indentations
called wells will be present, into which DNA samples can be loaded.)
The lab technician has prepared liquid gel for you. To make this solution, she first drew up a 3%
agarose solution by mixing dry agarose power with buffer solution. Then she heated the agarose
solution to dissolve the agarose powder.
Our lab tech drew up 1 liter of 3% (g/100 mL) agarose solution. How many grams of agarose
powder did she measure out to prepare this solution?
How many mL of buffer did she measure out to prepare the solution?
3. Allow at least 15 min for gel to cure (solidify).
4. While the gel cures, practice using the micropipette to load 15 uL samples in the practice gel
your instructor has set out for the class. You need to be very careful that you do not puncture the
well with the pipet tip when you load samples.
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