On a particular microscope, the numerical apertures of the condenser and the high dry power objective lenses are 1.25 and 0.65 respectively. You are supplied with a filter that selects a wavelength of 500 nm. A) What is the limit of resolution on this microscope when using the high dry power lens? (show your calculations) B) Will you be able to distinguish two points that are 260 nm apart as being separate or will they blur into one? (explain your answer)

On a particular microscope, the numerical apertures of the condenser and the high dry power objective lenses are 1.25 and 0.65 respectively. You are supplied with a filter that selects a wavelength of 500 nm. A) What is the limit of resolution on this microscope when using the high dry power lens? (show your calculations) B) Will you be able to distinguish two points that are 260 nm apart as being separate or will they blur into one? (explain your answer)

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter11: Filtration

Section: Chapter Questions

Problem 5RQ

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