Q: List two methods of high level disinfection.
A: The major difference between sterilization and disinfection is that sterilization involves the…
Q: What is a mixed culture? A contaminated culture?
A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: If an unknown bacteria was given that was gram-positive coccus, and the catalase test was performed,…
A: Gram-positive bacteria, such as Staphylococcus aureus, are bacteria that provide a positive response…
Q: how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.
A: Introduction Coliform is the gram-negative, non-spore-forming and motile or non-motile bacterial…
Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…
A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.
Q: Thermotolerant coliforms are often referred to as "faecal coliforms", although the organisms may not…
A: Microbes particularly bacteria occurs in diverse areas in the environment. They are of different…
Q: What is the logic behind testing for E. coli to detect fecalcontamination of water?
A: Bacteria are unicellular prokaryotic organism that maintains a definite shape since it contains the…
Q: Are the large numbers of microorganisms found in the mouth cause for concern? Explain.
A: We are authorized to answer one question at a time.Since you have not mentioned which one,I am…
Q: What is the media used in Catalase test and its expected results?
A: Catalase is an enzyme that is found in the cell in most of the organism that protect the cell from…
Q: If coliforms were present on a plated sample, would you know with certainty that the coliforms are…
A: Coliform microbes are characterized as rod-shaped, gram-negative, non-spore-forming, and…
Q: Why do we test water for coliforms if they are not considered pathogenic?
A: Introduction Coliforms are gram-negative, non-sporulating and motile or immotile bacteria. They…
Q: Which of the following water quality assay methods produce the greatest amount of biohazardous…
A: Introduction A biological hazard, often known as a biohazard, is a biological substance that…
Q: Why is glucose not the choice of carbohydrate for most differential media used in the identification…
A: Bacilli present in the intestinal tract are rod shaped bacteria.for example, enteric bacteria…
Q: Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features…
A: Staphylococcus aureus and Streptococcus pyogenes are gram positive bacteria that cause infections in…
Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…
A: A growth medium is a also known as a culture medium which can be solid, liquid, and even…
Q: After a series of experiments, bacterial cultures were identified for disposal. How can these be…
A: Decontamination refers to a process which reduces the biohazardous effects of an infectious agent,…
Q: If a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium…
A: Mannitol salt agar (MSA) is used as a culture medium or growth medium. Growth medium is a medium…
Q: Why is CFU more applicable to a culture of Streptococcus than to a culture of E. coli?
A: Colony-forming unit (CFU) represents the number of colonies formed on the media plate from one…
Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…
A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…
Q: Why do we use the term CFU (colony forming unit) instead of reporting bacteria per ml? Explain.
A: The bacterial species are responsible for causing several diseases within the human body. So, for…
Q: In what ways is the membrane filter method for coliform detection superior to the most probable…
A: Coloriform are group of bacteria present in the feces of several warm-blooded animals and human.…
Q: You were given a mixed nutrient agar broth culture of bacteria 1a. How will you determine the…
A: A mixed bacterial culture consists of more than one bacterial cell types growing together. On the…
Q: If a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium…
A: Manitol Salt Agar (MSA) in used in lab, mostly for selective and differential growth of microbial…
Q: not test water samples directly for Salmonella typhosa or other pathogens
A: There are serious limitations to the use of direct isolation of specific pathogenic bacteria for…
Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…
A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…
Q: can the phenol red test also be used to determine if a certain bacterium can metabolze various…
A: Differential media is a type of growth media used in the labs to grow and differentiate or…
Q: If the five-digit code that is tabulated cannot be found in the EnteroPluri-Test Codebook, what that…
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: Why is the incubation temperature criticalwhen using the Filter Method when testing water samples?
A: Incubation temperature is critical for bacterial growth if this temperature is too high or too low…
Q: What is the media used in Urease Test and expected results?
A: Urease test is defined as a biochemical test that distinguishes the basic alkaline fermentation of…
Q: In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For…
A:
Q: If you did the multiple-tube technique, list one advantage and one disadvantage of each method of…
A: Water from various sources obtained for routine testing were analyzed by Swedish standard methods…
Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…
A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…
Q: Do the results from a confirmed coliform test represent total or faecal coliform and why?
A: Fecal coliform bacteria may occur in ambient water as a result of the overflow of domestic sewage or…
Q: What microorganisms other than coliforms are likely to give positive presumptive test?
A:
Q: list the reagents used in the IMViC tests and what they detect
A: IMViC is a series of tests that include the indole test, methyl red test, Voges-Proskauer test, and…
Q: What kinds of organisms may be plentiful in a milk sample, yet give a negative reductase test?
A: Methylene blue reduction test is based on the principle of oxygen reduction by increased microbial…
Q: If testing had come back as oxidase negative, urease positive and growth on MacConkey agar, what…
A: MacConkey agar is a selective and differential culture medium used for the isolation and…
Q: Which one of the following methods of antimicrobial susceptibility testing combines some of the…
A: Antimicrobial susceptibility tests are performed to identify which drugs are effective against a…
Q: Calculate the CFU/ml of a urine sample if 49 coli colonies were counted on NA when 50 microliters…
A: In both basic and applied research, microbiology is a highly significant area. Proteomics, genomics,…
Q: You are testing for Escherichia coli in the sample. What media would you use? and Describe typical…
A: A medium in microbiology is defined as a solid or liquid substance used to culture the…
Q: If the seven-digit profile that is tabulated cannot be found in the API® 20E Analytical Profile…
A: The API® 20E System, is a biochemical panel for identifying and differentiating members of the…
Q: Describe the process of qualitative water test for coliform contamination. If shown EMB plates, be…
A: * Coliform bacteria are Bacteria and stains Gram negative and they can be motile or non motile…
Q: If the five-digit code that is tabulated cannot be found in the EnteroPluri-Test Codebook, what…
A: Introduction: EnteroPluri-Test is an identification system that offers complete identification of…
Q: In the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar…
A:
Q: Clear tubes in the MIC test were inoculated in Mueller-Hinton Agar to determine the MBC of the…
A: MBC - stands for minimum bactericidal concentration It is the lowest concentration of antibiotics…
Q: You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture.…
A: Answer : First step of the kirby-Bauer test :-
Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?
A: Inoculation is defined as the process of introduction of microorganisms into a culture for their…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
Q: What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or…
A: Staphylococcus Bacteria which grows in cluster are Gram-positive (catalase-positive). Streptococcus…
Once the completed test establishes the presence of coliforms in the water sample, why might you perform the IMViC tests on these isolates?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- When using Endo agar as the selective and differential media for the confirmed test, how would you know whether the test is positive for the presence of coli?What would be the expected out come of a Nitrate reduction test If the bacteria is a pathogenic intestinal bacteria?Why do the results for the confirmed test represent faecal coliform in the analysis of water using the MPN procedure?
- You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture. What is the first step to prepare your Kirby-Bauer test?Why is water tested for coliform bacteria rather than for pathogenic bacteria which may be present?What is the purpose of the confirmed test in an experiment designed to test for coliform bacteria?
- You are testing a river water sample. Your "original" plate shows 100 coliform colonies. Assuming you did the serial dilution correctly, how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.how to find out the test is differential or selective in the microbiology lab test?Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agar