One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression. Answer question A and B in order to answer the attached image questions Poll 1 and 2. A. You are doing an experiment with E. coli, what is the decay mRNA decay rate? B. If the sample initially has 0.25 pico-moles or mRNA, how much will remain in the sample if you freeze it 3 minutes later? See attached image for next question. Answer the two green poll questions please!!
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression.
Answer question A and B in order to answer the attached image questions Poll 1 and 2.
A. You are doing an experiment with E. coli, what is the decay mRNA decay rate?
B. If the sample initially has 0.25 pico-moles or mRNA, how much will remain in the sample if you freeze it 3 minutes later?
See attached image for next question. Answer the two green poll questions please!!
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