One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression. A. You are doing an experiment with E. coli, what is the decay mRNA decay rate?

One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression. A. You are doing an experiment with E. coli, what is the decay mRNA decay rate?

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Description: Please see two attached images and answer the two green questions aka poll 1 and poll 2! One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends onthe solutionit is in, in situ the half life of mRNA

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter11: Genome Alterations: Mutation And Epigenetics

Section: Chapter Questions

Problem 10QP: If the coding region of a gene (the exons) contains 2,100 base pairs of DNA, would a missense...

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One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression. Answer question A and B in order to answer the attached image questions Poll 1 and 2. A. You are doing an experiment with E. coli, what is the decay mRNA decay rate? B. If the sample initially has 0.25 pico-moles or mRNA, how much will remain in the sample if you freeze it 3 minutes later? See attached image for next question. Answer the two green poll questions please!!
The length of a particular gene in human DNA, measured from the start site for transcription to the end of the protein-coding region, is 10,000 nucleotides, whereas the length of the mRNA produced from this gene is 4000 nucleotides. What is the most likely reason for this difference?
The following is a list of mutations that have beendiscovered in a gene that has more than 60 exons andencodes a very large protein of 2532 amino acids.Indicate whether or not each mutation could cause adetectable change in the size or the amount of mRNAand/or a detectable change in the size or the amountof the protein product. (Detectable changes in size oramount must be greater than 1% of normal values.)What kind of change would you predict?a. Lys576Val (changes amino acid 576 from lysineinto valine)b. Lys576Argc. AAG576AAA (changes codon 576 from AAG toAAA)d. AAG576UAGe. Met1Arg (at least two possible scenarios exist forthis mutation)f. promoter mutationg. one base pair insertion into codon 1841h. deletion of codon 779i. IVS18DS, G–A, + 1 (this mutation changes thefirst nucleotide in the eighteenth intron of the gene,causing exon 18 to be spliced to exon 20, thusskipping exon 19)j. deletion of the poly-A addition sitek. G-to-A substitution in the 5′ UTRl. insertion of 1000 base…
central dogma of molecular biology describe the flow of information in the cell.There are also exceptions to the central dogma. Which of the following examples is inconsistent with the flow of genetic information in the cell? a. from DNA to mRNA b.from mRNA to protien c.from protien to DNA d.from DNA to rRNA e. from DNA to tRNA
Since it was deciphered four decades ago, somehave claimed that the genetic code must be a frozen acci-dent, while others have argued that it was shaped by nat-ural selection. A striking feature of the genetic code is itsinherent resistance to the effects of mutation. For example,a change in the third position of a codon often specifies thesame amino acid or one with similar chemical properties.The natural code resists mutation more effectively (is lesssusceptible to error) than most other possible versions, asillustrated in Figure Q1–1. Only one in a million comput-er-generated “random” codes is more error-resistant thanthe natural genetic code. Does the extraordinary mutationresistance of the genetic code argue in favor of its origin asa frozen accident or as a result of natural selection? Explainyour reasoning.
Once transcribed, the length of the mRNA for gene X is usually 1000 nucleotides long in your favorite bacterial species. After mutagenizing the bacteria, you notice that the mRNA is 800 nucleotides long. Which of the following could be the mutated that would explain your observations? a mutation introducing a new rut site in gene X a mutation in the Shine-Dalgarno sequence a mutation in the polyA addition site a mutation in the operator
Which of the following mutations in the protein-coding region of a gene is more likely to lead to complete loss of function of the encoded protein: an insertion of six nucleotides or a deletion of two nucleotides? Briefly explain your answer.
As described earlier, DNA damage can cause deletion or insertion of base pairs. If a nucleotide base sequence of a coding region changes by any number of bases other than three base pairs, or multiples of 3, a frameshift mutation occurs. Depending on the location of the sequence change, such mutations can have serious effects. The following synthetic mRNA sequence codes for the beginning of a polypeptide: 5′-AUGUCUCCUACUGCUGACGAGGGAAGGAGGUGGCUUAUC-AUGUUU-3′ First, determine the amino acid sequence of the polypeptide. Then determine the types of mutation that have occurred in the following altered mRNA segments. What effect do these mutations have on the polypeptide products? a. 5′-AUGUCUCCUACUUGCUGACGAGGGAAGGAGGUGGCUUAUCA-UGUUU-3′ b. 5′-AUGUCUCCUACUGCUGACGAGGGAGGAGGUGGCUUAUCAU-GUUU-3′ c. 5′-AUGUCUCCUACUGCUGACGAGGGAAGGAGGUGGCCCUUAUC-AUGUUU-3′ d. 5′-AUGUCUCCUACUGCUGACGGAAGGAGGUGGCUUAUCAU-GUUU-3′
In relation to central dogma of molecular biology answer the following questions: The length of a particular gene in human DNA, measured from the start site for transcription to the end of the protein-coding region, is 10,000 nucleotides, whereas the length of the mRNA produced from this gene is 4000 nucleotides. What is the most likely reason for this difference?
A diagram of a gene is shown below. Normally, exons 1, 2, and 3 are present in the mature mRNA. (shown in attatched image) A mutation occurred at the 5’ splice site of exon 1 as shown by the star such that the spliceosome cannot bind to this location during mRNA processing of the pre-mRNA into the mature mRNA. Which of the following is/are a possible result of this mutation? (select all that apply) a)The pre-mRNA transcribed from this gene sequence will be longer than normal. b)The protein translated from this mRNA will be a different length than the normal protein. c)The mature mRNA will be longer. d)The mature mRNA will be shorter.
In a study of several families for differences in the sequence of a particular gene suspected to cause a disease. Resulting in the discovery of different mutations in the genes of some families. For each of the mutations, specify if it could change the size or the quantity of mRNA and/or protein product? What kind of change would you predict? Explain each answer briefly. d. AAG378UAG e. promoter mutation. f. one base-pair insertion into codon #765 g. deletion of whole codon #765 h. G-to-A substitution in the 5' UTR i. insertion of 100 base pairs into the fourth intron (this insertion does not alter splicing).
Consider the following portion of mRNA produced by the normal order of DNA nucleotides: 5’ – CUU AAA CCA GUU – 3’ a. What is the template DNA sequence that was used to synthesize this portion of mRNA? b. What is the amino acid order produced from this mRNA? c. Write the amino acid sequence if a mutation changes CUU to CAU. Is this likely to affect protein function?