Outline the steps by which the polymerase chainreaction (PCR) amplifies a specific region of a genome.

Q: Explain why exome sequencing can be almost as valuable as genome sequencing.

A: Whole genome sequencing refers to technique which used to analyze complete genome of an organism at…

Q: Describe the shotgun method for sequencing an entire genome.

A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…

Q: I am having issues with differentiating between the different types of PCRs and why the different…

A: The polymerase chain reaction (PCR) is a technique in which several million copies of the desired…

Q: DNA extraction from banana is being analyzed

A: The stringy substance that you see is DNA! It has been removed from the millions and millions of…

Q: Explain Proofreading by DNA polymerase.

A: A DNA polymerase is an enzyme that catalyze the synthesis of DNA molecules from nucleoside…

Q: Describe some of the challenges associated with faithfully copying and maintaining the genome of an…

A: Although the genetic variation is important for evolution, the survival of the individual demands…

Q: Define the term PCR.

A: Dr.Kary Mullis developed the polymerase chain reaction (PCR) in 1983. The enzyme utilised in this…

Q: Explain how PCR eventually generates a discrete-sized fragment from a much longer piece of DNA.

A: Polymerase chain response (PCR) is a technique broadly used to quickly make millions to billions of…

Q: Outline different methods of DNA sequencing.

A: The sequence of a DNA (deoxyribonucleic acid) molecule is the order in which deoxyribonucleotides,…

Q: Diagram the process by which restriction enzymes andDNA ligase are used to make recombinant…

A: Recombinant DNA technology allows addition of a eukaryotic gene into a bacterial plasmid vector.…

Q: Contrast how gene cloning is different betweenrecombinant technology and PCR.

A: Gene cloning: It is also known as molecular cloning, it refers to the process of isolation of a DNA…

Q: Compare and contrast the use of PCR and gene cloning for amplifying DNA fragments. What are the…

A: Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy of…

Q: Explain the process of DNA cloning, and why researchers clonecDNA

A: DNA cloning is molecular biology technique to make or synthesize identical copies of the DNA. The…

Q: Explain the steps of next generation sequencing technique  in detail

A: Sequencing is an integral part of genomics that deals with the extraction and arranging of the…

Q: Explain what linkers and adapters are used in recombinant DNA studies.

A: Restriction enzymes can either create sticky ends or blunt ends. Sticky ends are those ends that…

Q: Explain how DNA microarrays are used in the study ofgenomics.

A: DNA (deoxyribonucleic acid) is double helix, coil structure that is responsible for transfer of…

Q: process of overlap assembly in whole genome sequencing

A: Genome sequencing Genome sequencing or gene sequencing is a way of arranging nucleotides the n the…

Q: Explain how oligonucleotide primers in the Polymerase Chain Reaction work (PCR)

A: PCR or polymerase chain reaction is an in-vitro DNA amplification technique. It is used to amplify…

Q: Define about computer-automated high-throughput DNA sequencing ?

A: DNA sequencing is a commonly used method after the gel electrophoresis to determine the order of…

Q: Explain why PCR results in the amplification of a specific DNA sequence despite many competing…

A: PCR is a very powerful and practical research tool which uses sequencing and identification DNA…

Q: Compare the possible differences between a eukaryotic protein-encoding gene cloned by PCR and the…

A: Specific mRNAs corresponding to a gene of interest are identified and reverse transcribed to form…

Q: Describe how the sequencing or sizing of PCR productscan elucidate genotypes.

A: PCR (polymerase chain reaction) product refers to the fragments of DNA (deoxyribonucleic acid) of…

Q: What are the different types of PCR and their product concepts? Site and explain the comparison…

A: Introduction :- Polymerase Chain Reaction (PCR) is a molecular biology technique for making multiple…

Q: Describe CRISPR as a genetic tool with its functions .

A: CRISPR ( Clustered Regularly Interspersed Short Palindromic Repeats ) is a segment of prokaryotic…

Q: Briefly describe what CRISPR technology does

A: Gene is a particular segment of deoxyribose nucleic acid (DNA) that is responsible for the…

Q: List the steps of a PCR cycle

A: DNA contains inheritable segments that are called genes that contain instructions for protein…

Q: Explain and list down the protocols in doing or preparation of materials for polymerase chain…

A: Polymerase chain reaction (PCR) It is an in vitro technique that takes a specific sequence of DNA…

Q: Outline the steps in automated DNA sequencing via the dideoxy method.

A: DNA sequencing:   It is a method to find a sequence of nucleotides by using the DNA sequencing…

Q: Briefly describe what happens during each of the phases of PCR (denaturation, annealing, and…

A: The “polymerase chain reaction” (PCR) is a technique in which millions of copies of the target DNA…

Q: Make a table comparing the advantages and disadvantages of PCR and gene cloning for amplifying DNA…

A: PCR or polymerase chain reaction is a method by which small fragments of DNA are used as a template…

Q: Cite three examples of an application of PCR (polymerase chain reaction).

A: Three examples of an application of PCR (polymerase chain reaction) -  1. Amplification of the DNA /…

Q: describe one method for sequencing DNA and a method for determining RNA sequencing

A: It means to determine the primary structure of an unbranched biopolymer.

Q: Describe how PCR is used to amplify a specific gene from total cellular DNA.

A: The process of amplifying a specific DNA sequence containing a gene of interest is known as…

Q: Explain in detail types of DNA sequencing?

A: DNA sequencing is a technique which is used to determine the sequence of DNA nucleotides within the…

Q: Write in detail what is the difference between PCR and real time PCR?

A: Polymerase chain response is a technique broadly used to quickly make millions to billions of…

Q: Explain how reverse-transcriptase PCR is carried out.

A: The reverse transcriptase-polymerase chain reaction is used to identify the genome of many RNA…

Q: Two techniques used to make large amounts of a segment of DNA or gene of interest are: polymerase…

A: The two techniques used to make large amounts of a segment of DNA or gene of interest are:…

Q: What specifically will happen if DNA polymerase is inaccurate during DNA synthesis? Explain how this…

A: The DNA polymerases are found during the DNA replication; these are group of catalyze that can…

Q: Explain the steps in recombinant DNA technology or rDNA technology

A: The combining of DNA molecules from two distinct species that are put into a host organism to…

Q: Differentiate between a PCR cycle and step, and define the function of each of the three steps used…

A: Polymerase chain reaction or PCR is a technique applied on DNA. DNA is a polymer of multiple…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.