Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam as defined by three different competing ligands Total binding Non-specific binding Specific binding c.p.m. 120.67 Total binding c.p.m. 143.00 Total binding c.p.m. 138 Diazepam c.p.m. 110.67 Flunitrazepam 128.00 2416.00 Non-specific binding Specific binding c.p.m. c.p.m. 133.00 c.p.m. c.p.m. Midazolam 2393.67 Non-specific binding Specific binding c.p.m. 2398.67 % Specific binding 95.24310118 % Specific binding 94.36268068 % Specific binding 94.55978975 The receptor or drug target in this case was the rat GABAA receptor obtained from homogenised rat brain cortical tissue. Using the same radioligand binding technique, what other way could you look at ligand affinity to GABAA receptors without using GABAA receptors derived from homogenised tissue? What are the advantages/disadvantages to each strategy?
Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam as defined by three different competing ligands Total binding Non-specific binding Specific binding c.p.m. 120.67 Total binding c.p.m. 143.00 Total binding c.p.m. 138 Diazepam c.p.m. 110.67 Flunitrazepam 128.00 2416.00 Non-specific binding Specific binding c.p.m. c.p.m. 133.00 c.p.m. c.p.m. Midazolam 2393.67 Non-specific binding Specific binding c.p.m. 2398.67 % Specific binding 95.24310118 % Specific binding 94.36268068 % Specific binding 94.55978975 The receptor or drug target in this case was the rat GABAA receptor obtained from homogenised rat brain cortical tissue. Using the same radioligand binding technique, what other way could you look at ligand affinity to GABAA receptors without using GABAA receptors derived from homogenised tissue? What are the advantages/disadvantages to each strategy?
Chapter8: Introduction To Cpt Coding
Section: Chapter Questions
Problem 9MC
Related questions
Question
![x₂ x² A2 A
V
Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam
as defined by three different competing ligands
I
Total binding
c.p.m.
Total binding Non-specific binding Specific binding % Specific binding
c.p.m.
120.67
143.00
Total binding
c.p.m.
138
c.p.m.
English (United Kingdom)
110.67
Diazepam
Flunitrazepam
c.p.m.
133.00
128.00
Non-specific binding Specific binding
c.p.m.
Non-specific binding
c.p.m.
2416.00
c.p.m.
Midazolam
♫
2393.67
♥
Specific binding
c.p.m.
2398.67
95.24310118
átv A
% Specific binding
94.36268068
% Specific binding
The receptor or drug target in this case was the rat GABAA receptor obtained from
homogenised rat brain cortical tissue. Using the same radioligand binding technique,
what other way could you look at ligand affinity to GABAA receptors without using
GABAA receptors derived from homogenised tissue? What are the
advantages/disadvantages to each strategy?
94.55978975
Styles St
P
Focus
each strategy?
N
E
X](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F19fb65da-94b8-41a4-a1ee-990279e142c6%2F521fcfb4-ce8c-4d7c-8e97-8b4c792eb4f1%2F3tgiltf_processed.jpeg&w=3840&q=75)
Transcribed Image Text:x₂ x² A2 A
V
Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam
as defined by three different competing ligands
I
Total binding
c.p.m.
Total binding Non-specific binding Specific binding % Specific binding
c.p.m.
120.67
143.00
Total binding
c.p.m.
138
c.p.m.
English (United Kingdom)
110.67
Diazepam
Flunitrazepam
c.p.m.
133.00
128.00
Non-specific binding Specific binding
c.p.m.
Non-specific binding
c.p.m.
2416.00
c.p.m.
Midazolam
♫
2393.67
♥
Specific binding
c.p.m.
2398.67
95.24310118
átv A
% Specific binding
94.36268068
% Specific binding
The receptor or drug target in this case was the rat GABAA receptor obtained from
homogenised rat brain cortical tissue. Using the same radioligand binding technique,
what other way could you look at ligand affinity to GABAA receptors without using
GABAA receptors derived from homogenised tissue? What are the
advantages/disadvantages to each strategy?
94.55978975
Styles St
P
Focus
each strategy?
N
E
X
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