Phosphate buffer solution is purchased as a(n) 2x stock solution. The solution is more concentrated than the working stock. If tasked with making 200 mL of a 1x solution of PBS, how many mL of the stock solution would be needed? Report your answer in standard decimal notation rounded to one decimal place. How many microliters of original sample are required to produce a final dilution of 103 in a total volume of 20 mL? 1 microliter is 10-6 L or 103 mL. • The dilution is equal to M2/M1

Phosphate buffer solution is purchased as a(n) 2x stock solution. The solution is more concentrated than the working stock. If tasked with making 200 mL of a 1x solution of PBS, how many mL of the stock solution would be needed? Report your answer in standard decimal notation rounded to one decimal place. How many microliters of original sample are required to produce a final dilution of 103 in a total volume of 20 mL? 1 microliter is 10-6 L or 103 mL. • The dilution is equal to M2/M1

Introductory Chemistry For Today

8th Edition

ISBN:9781285644561

Author:Seager

Publisher:Seager

Chapter7: Solutions And Colloids

Section: Chapter Questions

Problem 7.39E

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A 1.250 g sample of cheese was subjected to a Kjeldahl analysis to determine the amount of protein. The sample was digested, the nitrogen is oxidized to NH₄⁺, and was then converted to NH₃ with NaOH, and distilled into a collection flask containing 50.00 mL of 0.1050 M HCl. The excess HCl is back titrated with 0.1175 M NaOH, this required 21.65 mL to reach the bromothymol blue end point. A. How many moles of N is present in the cheese sample? B. Report the %N of the cheese sample. C. Report the %protein of the cheese sample, assuming that there are 6.4 grams of protein for every gram of nitrogen
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